Wood H A
Boyce Thompson Institute at Cornell University, Ithaca, New York 14853, USA.
Virology. 1980 Sep;105(2):338-44. doi: 10.1016/0042-6822(80)90035-5.
A spontaneous mutant of the Autographa californica nuclear polyhedrosis virus which was deficient in viral occlusion body formation was studied in Trichoplusia ni tissue culture cells. Virus multiplication could be divided into four phases based on growth curves and the sequential appearance of 25 viral-induced proteins identified by pulse labeling and polyacrylamide gel electrophoresis. During the latent phase, five viral-induced proteins with molecular weights of 68, 47, 35, and 30 thousand (K) were detected. The synthesis of 17 additional viral-induced proteins with molecular weights ranging from 64 to 14K was detected during the nonoccluded virus (NOV) phase, 10 to 16 hr postinoculation (pi). During the transition from NOV to occluded virus formation, 16 to 34 hr pi, the synthesis of occlusion body matrix protein (33K) and two additional proteins (15.6 and 13K) was detected. During the occlusion phase of replication, after 34 hr pi, NOV synthesis ceased; however, the synthesis of the 25 viral-induced proteins continued. Posttranslational cleavage was not detected in pulse-chase experiments. The occlusion body-deficient mutant induced lower levels of occlusion body matrix protein synthesis than the wild type virus. The decrease in amount of occluded virions was accompanied by a corresponding increase in the number of NOV particles produced.
在粉纹夜蛾组织培养细胞中研究了苜蓿银纹夜蛾核型多角体病毒的一种自发突变体，该突变体缺乏病毒包涵体的形成。根据生长曲线以及通过脉冲标记和聚丙烯酰胺凝胶电泳鉴定出的25种病毒诱导蛋白的相继出现情况，病毒增殖可分为四个阶段。在潜伏期，检测到5种病毒诱导蛋白，其分子量分别为68、47、35和30千道尔顿（K）。在接种后（pi）10至16小时的非包涵体病毒（NOV）阶段，检测到另外17种病毒诱导蛋白的合成，其分子量范围为64至14K。在从NOV向包涵体病毒形成的转变过程中，即接种后16至34小时，检测到包涵体基质蛋白（33K）以及另外两种蛋白（15.6K和13K）的合成。在复制的包涵体阶段，接种后34小时后，NOV合成停止；然而，25种病毒诱导蛋白的合成仍在继续。在脉冲追踪实验中未检测到翻译后切割。与野生型病毒相比，缺乏包涵体的突变体诱导的包涵体基质蛋白合成水平较低。包涵体病毒粒子数量的减少伴随着产生的NOV颗粒数量相应增加。
