Basconcillo Libia Saborido, McCarry Brian E
Department of Chemistry, McMaster University, West Hamilton, Ontario, Canada.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 1;871(1):22-31. doi: 10.1016/j.jchromb.2008.06.041. Epub 2008 Jul 1.
Three protocols for fatty acid analysis in Sinorhizobium meliloti were improved by the addition of a number of standards/controls and a silylation step which allowed the determination of recoveries, extents of conversion of lipids to fatty acid methyl esters (FAMEs) and extents of side reactions. Basic hydrolysis followed by acid-catalyzed methylation and transmethylation with sodium methoxide, were the best for the analysis of 3-hydroxy- and cyclopropane fatty acids, respectively. A micro-scale, one-vial method that employed sodium methoxide/methanol was equally efficient and on a 1000-fold smaller scale than standard methods. Because this method avoids aqueous extractions, 3-hydroxybutanoic acid was detected as its trimethylsilyloxy methyl ester along with FAMEs.
通过添加多种标准品/对照品以及一个硅烷化步骤，改进了三种用于苜蓿中华根瘤菌脂肪酸分析的方案，该硅烷化步骤可用于测定回收率、脂质转化为脂肪酸甲酯（FAME）的程度以及副反应的程度。碱性水解后进行酸催化甲基化以及用甲醇钠进行转甲基化，分别是分析3-羟基脂肪酸和环丙烷脂肪酸的最佳方法。一种采用甲醇钠/甲醇的微量单瓶法同样高效，且规模比标准方法小1000倍。由于该方法避免了水相萃取，3-羟基丁酸与其脂肪酸甲酯一起被检测为其三甲基硅氧基甲酯。
