[荷瘤大鼠血浆皮质转运蛋白的色谱分析]
[Chromatographic transcortin analysis of the blood plasma in tumor-bearing rats].
作者信息
Krauialis K I, Diashrius A P, Ulinskaĭte A P, Kershulis A A
出版信息
Vopr Onkol. 1980;26(9):53-6.
The binding of 3H corticosterone was studied comparatively in partly purified preparations of blood plasma transcortin in intact and tumor-bearing rats at the 12th day after transplantation. By means of chromatography on DEAE-cellulose and hydroxylapatite transcortin was purified about 200 times. It was demonstrated that the content of bound 3H corticosterone in 1 mg of protein in blood plasma preparations from tumor-bearing animals is 3.5 times less than in the plasma of intact rats. The observed reduction of the transcortin binding capacity in the blood plasma of rats with Walker carcinosarcoma is retained also in purified preparations of the blood plasma tanscortin of tumor-bearing rats.
在移植后第12天，对完整大鼠和荷瘤大鼠血浆皮质素转运蛋白的部分纯化制剂中3H皮质酮的结合情况进行了比较研究。通过在DEAE - 纤维素和羟基磷灰石上进行色谱分离，皮质素转运蛋白被纯化了约200倍。结果表明，荷瘤动物血浆制剂中每1毫克蛋白质结合的3H皮质酮含量比完整大鼠血浆中的少3.5倍。在Walker癌肉瘤大鼠血浆中观察到的皮质素转运蛋白结合能力的降低，在荷瘤大鼠血浆皮质素转运蛋白的纯化制剂中也依然存在。
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