Liu Min, Zhu Kai-ling, Li Hong-bo, Zhang Tao, Xiao Tian
Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Huan Jing Ke Xue. 2008 Apr;29(4):1082-91.
Bacterial community composition and predominant bacteria were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) in the Yellow Sea Cold Water Mass (YSCWM). The results analysed by Glyko Bandscan software showed that samples from all stations in April and stations in the YSCWM area in October had almost the same number of different DNA bands (17 and 16 respectively ) with high diversity. However, samples from the stations out of YSCWM area had fewer DNA bands (about 12) with lower diversity. 24 DGGE bands reflecting varying phylotypes were excised, cloned and sequenced. Phylogenetic analysis of 24 cloned bands showed that bacterial phylotypes were made up of two phyla bacteria, Proteobacteria and Bacteroides. Among these sequences, 16 were related to gamma-Proteobacteria and delta-Proteobacteria and 5 were related to Bacteroides. It indicated that bacterial community composition, as well as the predominant bacterial groups, from all sampling stations in April (the temperature of seawater was around 7-12 degrees C) and the inter of YSCWM in October (< or = 10 degrees C) was the same, different from the exterior of YSCWM (> or = 19 degrees C). By comparing, from samples of exterior of YSCWM, the predominant bacteria groups were different with the different sampling stations. So it suggested that bacterial community composition and the characteristic of predominant bacteria of the YSCWM may have something to do with the development of the YSCWM.
采用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）技术对黄海冷水团（YSCWM）中的细菌群落组成和优势菌进行了分析。经Glyko Bandscan软件分析的结果显示，4月份所有站位以及10月份黄海冷水团区域内站位的样本具有几乎相同数量的不同DNA条带（分别为17条和16条），多样性较高。然而，黄海冷水团区域外站位的样本DNA条带较少（约12条），多样性较低。切除了反映不同系统发育型的24条DGGE条带，进行克隆和测序。对24个克隆条带的系统发育分析表明，细菌系统发育型由变形菌门和拟杆菌门两个门类的细菌组成。在这些序列中，16条与γ-变形菌纲和δ-变形菌纲相关，5条与拟杆菌相关。这表明4月份（海水温度约为7-12摄氏度）所有采样站位以及10月份黄海冷水团内部（≤10摄氏度）的细菌群落组成以及优势细菌类群相同，与黄海冷水团外部（≥19摄氏度）不同。通过比较发现，在黄海冷水团外部的样本中，不同采样站位的优势细菌类群不同。因此表明，黄海冷水团的细菌群落组成和优势菌特征可能与黄海冷水团的形成有关。
