Bai Jie, Li Haiyan, Zhao Yangguo
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Wei Sheng Wu Xue Bao. 2009 Mar;49(3):343-50.
To reveal the diversity of marine bacteria in disparate sites of the Northern Yeallow Sea.
Bacterial community structure and diversity within seawaters and sediments at two stations in the Northern Yellow Sea were investigated and assessed by the 16S rDNA clone library and the denaturing gradient gel electrophoresis (DGGE) techniques.
The 16S rDNA clone library analysis indicated that the bacteria were abundant in the waters and sediments, and most of them were unknown. Phylogenetic analysis showed that the Proteobacteria were the dominant group both in the sediments and waters, gamma-Proteobacteria and delta-Proteobacteria were dominant in the sediments, alpha-proteobacteria were dominant in the water. However, all subphyla of Proteobacteria presented phylogenetical divergence at the two sites. The clustering analysis on the DGGE patterns revealed that the dominant groups within the waters and sediments in shore were similar to each other, whereas that in the offshore were different.
The microbial diversity differed due to the geographical location and living medium; and the microbial distribution depended on the environment factors.
揭示北黄海不同地点海洋细菌的多样性。
采用16S rDNA克隆文库和变性梯度凝胶电泳（DGGE）技术，对北黄海两个站点海水和沉积物中的细菌群落结构及多样性进行调查和评估。
16S rDNA克隆文库分析表明，海水和沉积物中细菌种类丰富，大部分为未知细菌。系统发育分析显示，变形菌门在沉积物和海水中均为优势菌群，γ-变形菌纲和δ-变形菌纲在沉积物中占优势，α-变形菌纲在海水中占优势。然而，变形菌门的所有亚门在两个站点均呈现出系统发育差异。对DGGE图谱的聚类分析表明，近岸海水和沉积物中的优势菌群彼此相似，而离岸的则不同。
微生物多样性因地理位置和生存介质而异；微生物分布取决于环境因素。
