Margis-Pinheiro M, Metz-Boutigue M H, Awade A, de Tapia M, le Ret M, Burkard G
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Plant Mol Biol. 1991 Aug;17(2):243-53. doi: 10.1007/BF00039499.
The amino acid sequences of peptides generated by trypsin and chymotrypsin digestions of the acidic PR4 chitinase from bean were determined. Oligonucleotide primers derived from this sequence were used to synthesize a PR4 chitinase-specific probe by PCR-amplification. This probe allowed the isolation of cDNA clones encoding PR4 chitinase that have been sequenced. This acidic and extracellular chitinase shows some homology to the basic isoform from the same plant, and differs from other known acidic chitinases by the presence of an amino-terminal cysteine-rich domain. Southern blot analysis of bean genomic DNA revealed that PR4 chitinase is encoded by a single gene.
测定了菜豆酸性PR4几丁质酶经胰蛋白酶和胰凝乳蛋白酶消化产生的肽段的氨基酸序列。根据该序列设计的寡核苷酸引物用于通过聚合酶链反应（PCR）扩增合成PR4几丁质酶特异性探针。该探针用于分离已测序的编码PR4几丁质酶的cDNA克隆。这种酸性胞外几丁质酶与同一植物的碱性同工型具有一定同源性，并且由于存在富含氨基末端半胱氨酸的结构域而与其他已知的酸性几丁质酶不同。菜豆基因组DNA的Southern杂交分析表明，PR4几丁质酶由单基因编码。
