Broglie K E, Gaynor J J, Broglie R M
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6820-4. doi: 10.1073/pnas.83.18.6820.
A full-length copy of bean leaf chitinase mRNA has been cloned. The 1146-base-pair insert of pCH18 encodes the 27-residue amino-terminal signal peptide of the precursor and 301 residues of the mature protein. Utilizing pCH18 as a hybridization probe, we have shown that the increase in translatable chitinase mRNA seen upon ethylene treatment of bean seedlings is due to a 75- to 100-fold increase in steady-state mRNA levels. Southern blot analysis of bean genomic DNA revealed that chitinase is encoded by a small, multigene family consisting of approximately four members. From our nucleotide sequence analysis of five additional chitinase cDNA clones, it appears that at least two of these genes are expressed. Three of the bean chitinase genes have been isolated from a Sau3A genomic library and partially characterized.
已克隆出菜豆叶几丁质酶mRNA的全长拷贝。pCH18中1146个碱基对的插入片段编码前体的27个氨基酸的氨基末端信号肽和成熟蛋白的301个氨基酸。利用pCH18作为杂交探针，我们发现菜豆幼苗经乙烯处理后可翻译的几丁质酶mRNA增加，这是由于稳态mRNA水平增加了75至100倍。对菜豆基因组DNA的Southern印迹分析表明，几丁质酶由一个小的多基因家族编码，该家族约由四个成员组成。通过对另外五个几丁质酶cDNA克隆的核苷酸序列分析，似乎这些基因中至少有两个是表达的。已从Sau3A基因组文库中分离出三个菜豆几丁质酶基因并进行了部分表征。
