Ishige F, Mori H, Yamazaki K, Imaseki H
Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Japan.
Plant Cell Physiol. 1993 Jan;34(1):103-11.
A complementary DNA encoding an ethylene-inducible acidic chitinase of azuki bean (Vigna angularis) was isolated, and its complete nucleotide sequence was determined. The nucleotide and deduced amino-acid sequence were very similar to those of an acidic chitinase from cucumber leaves that had been infected with tobacco necrosis virus. The mRNA for the acidic chitinase was not detected in leaves of azuki bean that had not been treated with ethylene, but it appeared 3 h after initiation of treatment with ethylene and its level gradually increased over a period of 19 h. The mRNA also accumulated in response to salicylate or wounding. The expression of the gene in response to wounding was suppressed by 2,5-norbornadiene, but that in response to salicylate was not affected by this inhibitor.
分离出了编码小豆（Vigna angularis）乙烯诱导酸性几丁质酶的互补DNA，并确定了其完整的核苷酸序列。该核苷酸和推导的氨基酸序列与感染烟草坏死病毒的黄瓜叶片中的酸性几丁质酶非常相似。在未用乙烯处理的小豆叶片中未检测到酸性几丁质酶的mRNA，但在用乙烯处理开始后3小时出现，其水平在19小时内逐渐增加。mRNA也响应水杨酸或创伤而积累。该基因对创伤的响应表达受到2,5-降冰片二烯的抑制，但对水杨酸的响应不受该抑制剂的影响。
