Kong Wei-Jun, Zhao Yan-Ling, Shan Li-Mei, Xiao Xiao-He, Guo Wei-Ying
Institute of Chinese Materia Medica, 302 Hospital of People's Liberation Army, Beijing, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 1;871(1):109-14. doi: 10.1016/j.jchromb.2008.06.053. Epub 2008 Jul 6.
This work investigated the spectrum-effect relationships between HPLC fingerprints and the anti-bacterial activities of EtOAc extracts from Radix Isatidis. Fingerprints of EtOAc extracts of Radix Isatidis from various sources were established by a High-Performance Liquid Chromatography. The process of Escherichia coli (E. coli) growth affected by EtOAc extracts was monitored using a Thermal Activity Monitor (TAM) Air Isothermal Calorimeter by microcalorimetry. By analyzing the power-time curves, quantitative parameters, such as growth rate constant k, maximum heat-production rate P(m), appearance time t and total heat-production Q were obtained to characterize the interactions of E. coli and the EtOAc extracts from Radix Isatidis. The HPLC fingerprints were investigated using hierarchical clustering analysis. The main thermo-kinetic parameters from the power-time curves were analyzed using principal component analysis. The spectrum-effect relationships between the HPLC fingerprints and anti-bacterial activities were analyzed with multivariant correlation analysis. Close correlation existed between the spectrum-effect relationships of the EtOAc extracts. Salicylic acid in the HPLC fingerprints might be one of the anti-bacterial components. This work provides a general model of the combination of HPLC and microcalorimetry to study the spectrum-effect relationships of EtOAc extracts from Radix Isatidis, which can be used to search for principal components of Radix Isatidis on bioactivity.
本研究考察了板蓝根乙酸乙酯提取物的高效液相色谱（HPLC）指纹图谱与其抗菌活性之间的谱效关系。采用高效液相色谱法建立了不同来源板蓝根乙酸乙酯提取物的指纹图谱。使用热活性监测仪（TAM）空气等温量热仪通过微量量热法监测乙酸乙酯提取物对大肠杆菌（E. coli）生长过程的影响。通过分析功率-时间曲线，获得了生长速率常数k、最大产热速率P(m)、出现时间t和总产热量Q等定量参数，以表征大肠杆菌与板蓝根乙酸乙酯提取物之间的相互作用。采用层次聚类分析法对HPLC指纹图谱进行研究。运用主成分分析法对功率-时间曲线的主要热动力学参数进行分析。采用多元相关性分析方法分析HPLC指纹图谱与抗菌活性之间的谱效关系。乙酸乙酯提取物的谱效关系之间存在密切相关性。HPLC指纹图谱中的水杨酸可能是抗菌成分之一。本研究提供了一种HPLC与微量量热法相结合的通用模型，用于研究板蓝根乙酸乙酯提取物的谱效关系，可用于寻找板蓝根具有生物活性的主要成分。
