Mogensen Jesper, Boyd Mette Hjarsø, Nielsen Mai Drost, Kristensen Roar Solholt, Malá Hana
The Unit for Cognitive Neuroscience, Department of Psychology, University of Copenhagen, Denmark.
Brain Res Bull. 2008 Sep 5;77(1):1-7. doi: 10.1016/j.brainresbull.2008.05.005. Epub 2008 Jun 20.
Systemically administered human recombinant erythropoietin (EPO) may have the potential to reduce the cognitive and behavioural symptoms of mechanical brain injury. In a series of studies we address this possibility. Previously, we studied the effects of EPO given to fimbria-fornix transected rats at the moment of injury. We have found that such treatment improves substantially the posttraumatic acquisition of allocentric place learning tasks administered in a water maze and in an 8-arm radial maze as well as a spatial delayed alternation task administered in a T-maze. It is, however, essential also to evaluate this clinically important ability of EPO after other types of mechanical brain injury. Consequently, we presently studied the effects of similarly administered EPO in rats subjected to bilateral subpial aspiration of the anteromedial prefrontal cortex as well as control operated rats, respectively. We evaluated the posttraumatic behavioural/cognitive abilities of these animals in a spatial delayed alternation task performed in a T-maze. Administration of EPO to the prefrontally ablated rats was associated with a reduction of the lesion-associated behavioural impairment--while such an impairment was clearly seen in the saline injected prefrontally ablated group. In sham operated rats administration of EPO did not influence the task acquisition significantly. The results of the present study confirm our previous demonstrations that EPO is able to reduce the behavioural/cognitive consequences of mechanical brain injury. This ability is emphasized by its relative independence on the type of lesion as well as the neural structure affected.
全身给药的人重组促红细胞生成素（EPO）可能具有减轻机械性脑损伤的认知和行为症状的潜力。在一系列研究中，我们探讨了这种可能性。此前，我们研究了在损伤时刻给穹窿海马伞横断大鼠注射EPO的效果。我们发现，这种治疗显著改善了在水迷宫和八臂放射状迷宫中进行的以自我为中心的位置学习任务的创伤后习得，以及在T迷宫中进行的空间延迟交替任务。然而，在其他类型的机械性脑损伤后评估EPO的这种临床重要能力也很重要。因此，我们目前分别研究了在双侧软膜下抽吸前额内侧皮质的大鼠以及对照手术大鼠中给予类似剂量EPO的效果。我们在T迷宫中进行的空间延迟交替任务中评估了这些动物创伤后的行为/认知能力。给前额叶切除的大鼠注射EPO与损伤相关的行为损伤减少有关——而在注射生理盐水的前额叶切除组中明显可见这种损伤。在假手术大鼠中，注射EPO对任务习得没有显著影响。本研究结果证实了我们之前的论证，即EPO能够减轻机械性脑损伤的行为/认知后果。这种能力因其相对独立于损伤类型以及受影响的神经结构而得到强调。
