积累脯氨酸的自我克隆面包酵母可提高面团的抗冻性。
Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughs.
作者信息
Kaino Tomohiro, Tateiwa Tetsuya, Mizukami-Murata Satomi, Shima Jun, Takagi Hiroshi
机构信息
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
出版信息
Appl Environ Microbiol. 2008 Sep;74(18):5845-9. doi: 10.1128/AEM.00998-08. Epub 2008 Jul 18.
Abstract
We constructed self-cloning diploid baker's yeast strains by disrupting PUT1, encoding proline oxidase, and replacing the wild-type PRO1, encoding gamma-glutamyl kinase, with a pro1(D154N) or pro1(I150T) allele. The resultant strains accumulated intracellular proline and retained higher-level fermentation abilities in the frozen doughs than the wild-type strain. These results suggest that proline-accumulating baker's yeast is suitable for frozen-dough baking.
摘要
我们通过破坏编码脯氨酸氧化酶的PUT1，并将编码γ-谷氨酰激酶的野生型PRO1替换为pro1（D154N）或pro1（I150T）等位基因，构建了自克隆二倍体面包酵母菌株。所得菌株积累细胞内脯氨酸，并且在冷冻面团中比野生型菌株保留更高水平的发酵能力。这些结果表明，积累脯氨酸的面包酵母适用于冷冻面团烘焙。
相似文献
1
Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughs.
Appl Environ Microbiol. 2008 Sep;74(18):5845-9. doi: 10.1128/AEM.00998-08. Epub 2008 Jul 18.
2
Isolation of baker's yeast mutants with proline accumulation that showed enhanced tolerance to baking-associated stresses.
Int J Food Microbiol. 2016 Dec 5;238:233-240. doi: 10.1016/j.ijfoodmicro.2016.09.015. Epub 2016 Sep 22.
3
Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast.
Microb Cell Fact. 2012 Apr 1;11:40. doi: 10.1186/1475-2859-11-40.
4
Simultaneous accumulation of proline and trehalose in industrial baker's yeast enhances fermentation ability in frozen dough.
J Biosci Bioeng. 2012 May;113(5):592-5. doi: 10.1016/j.jbiosc.2011.12.018. Epub 2012 Jan 26.
5
Proline accumulation in baker's yeast enhances high-sucrose stress tolerance and fermentation ability in sweet dough.
Int J Food Microbiol. 2012 Jan 3;152(1-2):40-3. doi: 10.1016/j.ijfoodmicro.2011.10.004. Epub 2011 Oct 14.
6
Improving freeze-tolerance of baker's yeast through seamless gene deletion of NTH1 and PUT1.
J Ind Microbiol Biotechnol. 2016 Jun;43(6):817-28. doi: 10.1007/s10295-016-1753-7. Epub 2016 Mar 10.
7
Development of intra-strain self-cloning procedure for breeding baker's yeast strains.
J Biosci Bioeng. 2017 Mar;123(3):319-326. doi: 10.1016/j.jbiosc.2016.10.008. Epub 2016 Nov 6.
8
Antioxidant N-acetyltransferase Mpr1/2 of industrial baker's yeast enhances fermentation ability after air-drying stress in bread dough.
Int J Food Microbiol. 2010 Mar 31;138(1-2):181-5. doi: 10.1016/j.ijfoodmicro.2010.01.001. Epub 2010 Jan 11.
9
L-proline accumulation and freeze tolerance of Saccharomyces cerevisiae are caused by a mutation in the PRO1 gene encoding gamma-glutamyl kinase.
Appl Environ Microbiol. 2003 Jan;69(1):212-9. doi: 10.1128/AEM.69.1.212-219.2003.
10
Frozen-dough baking potential of psychrotolerant Saccharomyces species and derived hybrids.
Food Microbiol. 2021 Apr;94:103640. doi: 10.1016/j.fm.2020.103640. Epub 2020 Sep 9.
引用本文的文献
1
Enhancing freeze-thaw tolerance in baker's yeast: strategies and perspectives.
Food Sci Biotechnol. 2024 Jul 3;33(13):2953-2969. doi: 10.1007/s10068-024-01637-6. eCollection 2024 Oct.
2
Proline Homeostasis in : How Does the Stress-Responsive Transcription Factor Msn2 Play a Role?
Front Genet. 2020 Apr 28;11:438. doi: 10.3389/fgene.2020.00438. eCollection 2020.
3
Proline metabolism regulates replicative lifespan in the yeast .
Microb Cell. 2019 Sep 24;6(10):482-490. doi: 10.15698/mic2019.10.694.
4
Quantitative Trait Nucleotides Impacting the Technological Performances of Industrial Strains.
Front Genet. 2019 Jul 23;10:683. doi: 10.3389/fgene.2019.00683. eCollection 2019.
5
Physiology, ecology and industrial applications of aroma formation in yeast.
FEMS Microbiol Rev. 2017 Aug 1;41(Supp_1):S95-S128. doi: 10.1093/femsre/fux031.
6
Improving industrial yeast strains: exploiting natural and artificial diversity.
FEMS Microbiol Rev. 2014 Sep;38(5):947-95. doi: 10.1111/1574-6976.12073. Epub 2014 May 8.
7
Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast.
Microb Cell Fact. 2012 Apr 1;11:40. doi: 10.1186/1475-2859-11-40.
8
Glutamate kinase from Thermotoga maritima: characterization of a thermophilic enzyme for proline biosynthesis.
Extremophiles. 2010 Jul;14(4):409-15. doi: 10.1007/s00792-010-0320-9. Epub 2010 Jun 11.
9
Pyrroline-5-carboxylate synthase and proline biosynthesis: from osmotolerance to rare metabolic disease.
Protein Sci. 2010 Mar;19(3):372-82. doi: 10.1002/pro.340.
本文引用的文献
1
Gene expression profiles and intracellular contents of stress protectants in Saccharomyces cerevisiae under ethanol and sorbitol stresses.
Appl Microbiol Biotechnol. 2008 May;79(2):273-83. doi: 10.1007/s00253-008-1431-4.
2
Construction and analysis of self-cloning sake yeasts that accumulate proline.
J Biosci Bioeng. 2007 Apr;103(4):377-80. doi: 10.1263/jbb.103.377.
3
Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance.
Appl Environ Microbiol. 2007 Jun;73(12):4011-9. doi: 10.1128/AEM.00730-07. Epub 2007 Apr 20.
4
Trehalose synthesis in Saccharomycopsis fibuligera does not respond to stress treatments.
Appl Microbiol Biotechnol. 2007 Apr;74(5):1084-91. doi: 10.1007/s00253-006-0688-8. Epub 2006 Nov 4.
5
Industrial Application of Artificially Induced Diploid Strains of Torulaspora delbrueckii.
Appl Environ Microbiol. 1987 Jul;53(7):1512-4. doi: 10.1128/aem.53.7.1512-1514.1987.
6
Induction and Characterization of Artificial Diploids from the Haploid Yeast Torulaspora delbrueckii.
Appl Environ Microbiol. 1987 Jul;53(7):1504-11. doi: 10.1128/aem.53.7.1504-1511.1987.
7
Selection of yeasts for breadmaking by the frozen-dough method.
Appl Environ Microbiol. 1986 Oct;52(4):941-3. doi: 10.1128/aem.52.4.941-943.1986.
8
Effect of L-proline on sake brewing and ethanol stress in Saccharomyces cerevisiae.
Appl Environ Microbiol. 2005 Dec;71(12):8656-62. doi: 10.1128/AEM.71.12.8656-8662.2005.
9
Effect of proline and arginine metabolism on freezing stress of Saccharomyces cerevisiae.
J Biosci Bioeng. 2002;94(5):390-4. doi: 10.1016/s1389-1723(02)80214-6.
10
Glucose and sucrose: hazardous fast-food for industrial yeast?
Trends Biotechnol. 2004 Oct;22(10):531-7. doi: 10.1016/j.tibtech.2004.08.001.
Zotero 插件