Osterberg E, Hallander H O, Kallner A, Lundin A, Aberg H
Department of Family Medicine, Karolinska Institute, Stockholm, Sweden.
Eur J Clin Microbiol Infect Dis. 1991 Feb;10(2):70-3. doi: 10.1007/BF01964410.
Determination by bioluminescence of the bacterial adenosine triphosphate (ATP) level in urine was evaluated as a method for detection of bacteriuria in 1126 women with symptoms of UTI and 530 attending for follow-up. Conventional urine culture was used as reference method. The criterion for bacteriuria was growth of greater than or equal to 10(5) cfu/ml, giving a prevalence of 0.60. ATP levels of less than 10 nmol/l and greater than 50 mmol/l indicated abacteriuria and bacteruiria, respectively, whereas intermediate concentrations required culture if the nitrite test was negative. With this diagnostic strategy the sensitivity and specificity was 0.96 and 0.90 at the first visit and 0.90 and 0.98 at follow-up. With some methodological improvement the ATP test could be useful in medium-sized and small laboratories.
通过生物发光法测定尿液中细菌三磷酸腺苷（ATP）水平，以此作为检测1126名有尿路感染症状女性和530名前来复诊女性菌尿症的一种方法进行评估。传统尿液培养用作参考方法。菌尿症的标准是菌落形成单位（cfu）/毫升大于或等于10⁵，患病率为0.60。ATP水平低于10纳摩尔/升和高于50毫摩尔/升分别表明无菌尿症和菌尿症，而如果亚硝酸盐试验为阴性，中间浓度则需要进行培养。采用这种诊断策略，首次就诊时的敏感性和特异性分别为0.96和0.90，复诊时为0.90和0.98。经过一些方法学改进，ATP检测在中小型实验室可能会有用。
