光系统II产生的过氧化氢参与光抑制作用。

The involvement of photosystem II-generated H2O2 in photoinhibition.

作者信息

Bradley R L, Long K M, Frasch W D

机构信息

Department of Botany, Arizona State University, Tempe 85287-1601.

出版信息

FEBS Lett. 1991 Jul 29;286(1-2):209-13. doi: 10.1016/0014-5793(91)80975-9.

DOI:10.1016/0014-5793(91)80975-9

PMID:1864370

Abstract

The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibitory illumination. Photoinhibition was accelerated for both Cl(-)-depleted and Cl(-)-sufficient thylakoids when KCN was present to inhibit the thylakoid-bound Fe-catalase. We propose that these preparations become photoinhibited by reactions with H2O2 produced via oxidation of water by the Cl(-)-depleted OEC and by reduction of O2 at the QB site when PSII is illuminated without an electron acceptor.

摘要

研究了光系统II（PSII）产生的H2O2在具有功能性放氧复合体（OEC）的类囊体光抑制过程中的作用。当在光抑制光照期间存在牛铁催化酶时，光抑制速率降低至黑暗中活性丧失的速率。当存在KCN抑制类囊体结合的铁催化酶时，Cl(-)耗尽和Cl(-)充足的类囊体的光抑制均加速。我们提出，当PSII在没有电子受体的情况下受到光照时，这些制剂会通过与Cl(-)耗尽的OEC氧化水产生的H2O2反应以及在QB位点还原O2而受到光抑制。