Liu F F, Li G P, Li H W
College of Life Sciences, Huaibei Normal University, 235000 Huaibei, China.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China.
Photosynthetica. 2023 Mar 30;61(1):97-107. doi: 10.32615/ps.2023.008. eCollection 2023.
Deg proteases play critical roles in photoprotection and PSII-repair circle, which remains elusive in cereal crops including wheat. Here, a Deg7-encoding gene was silenced in wheat a -induced gene-silencing system (BSMV-VIGS). When the expression level of was downregulated, the photosynthetic activity including CO assimilation rate, actual photochemical efficiency of PSII, and electron transport rate declined while the nonphotochemical quenching increased significantly. When grown in high light, the plants accumulated more soluble sugar, malondialdehyde, and superoxide anion but had lower superoxide dismutase activity and less ascorbic acid. Additionally, the expression levels of and were repressed in the plants in high light. The plants also were more sensitive to high-light stress. Collectively, it appeared that may be a potential target for wheat radiation-use efficiency improvement against high light stress.
Deg蛋白酶在光保护和光系统II修复循环中发挥关键作用，这在包括小麦在内的谷类作物中仍不清楚。在此，利用大麦条纹花叶病毒诱导的基因沉默系统（BSMV-VIGS）在小麦中沉默了一个编码Deg7的基因。当Deg7的表达水平下调时，光合活性包括CO2同化率、光系统II的实际光化学效率和电子传递速率下降，而非光化学猝灭显著增加。在高光条件下生长时，Deg7沉默的植株积累了更多的可溶性糖、丙二醛和超氧阴离子，但超氧化物歧化酶活性较低且抗坏血酸较少。此外，在高光条件下，Deg7沉默植株中PSII和LHCB1的表达水平受到抑制。Deg7沉默植株对高光胁迫也更敏感。总体而言，Deg7可能是提高小麦抗高光胁迫辐射利用效率的一个潜在靶点。
