通过用针对合成肽的抗体进行免疫沉淀来鉴定烟草花叶病毒的30K蛋白。

Identification of the 30K protein of TMV by immunoprecipitation with antibodies directed against a synthetic peptide.

作者信息

Ooshika I, Watanabe Y, Meshi T, Okada Y, Igano K, Inouye K, Yoshida N

机构信息

Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo 113, Japan.

出版信息

Virology. 1984 Jan 15;132(1):71-8. doi: 10.1016/0042-6822(84)90092-8.

DOI:10.1016/0042-6822(84)90092-8

PMID:18639799

Abstract

A synthetic hexadecapeptide corresponding to the predicted C-terminal sequence of the 30K protein of TMV has been coupled to bovine serum albumin and used to raise antibodies in rabbits. The resulting antiserum reacted with the 30K protein translated in vitro. We report the use of this antiserum in the first detection of the 30K protein in vivo, in TMV-infected tobacco protoplasts. Several proteins, the so called family of 30K-related peptides, were immunoprecipitated among in vitro translation products, but only the 30K protein was immunoprecipitated from TMV-infected protoplasts.

摘要

一种与烟草花叶病毒（TMV）30K蛋白预测的C端序列相对应的合成十六肽已与牛血清白蛋白偶联，并用于在兔体内产生抗体。所得抗血清与体外翻译的30K蛋白发生反应。我们报道了使用这种抗血清首次在体内检测TMV感染的烟草原生质体中的30K蛋白。在体外翻译产物中免疫沉淀出几种蛋白质，即所谓的30K相关肽家族，但从TMV感染的原生质体中仅免疫沉淀出30K蛋白。