Franzini-Armstrong C
Department of Anatomy, University of Pennsylvania, Philadelphia 19104-6058.
Dev Biol. 1991 Aug;146(2):353-63. doi: 10.1016/0012-1606(91)90237-w.
The development and maturation of transverse (T) tubules and sarcoplasmic reticulum (SR) have been studied in pre- and postnatal mouse muscle, using selective "staining" of these membrane systems. As previously reported in the literature, orderly transverse orientation of the T tubules occurs late in development and early T-SR junctions (triads and dyads) are located at random along the T tubules in a predominantly longitudinal orientation. We find that initial appearance of transverse tubules occurs fairly abruptly, and that all early T tubules have a longitudinal orientation. Transverse orientation of the T tubule network, location of triads at the A-I junction, and development of differentiated regions of the SR are coordinated events which occur gradually over a period of about 3 weeks for leg muscle.s The timing of triad development coincides with that reported for the increase in slow calcium current and dihydropyridine binding. Differences in T tubule patterns between muscle fibers of EDL and soleus are apparent only at relatively late stages.
利用这些膜系统的选择性“染色”方法，对出生前和出生后的小鼠肌肉中横管（T管）和肌浆网（SR）的发育及成熟过程进行了研究。正如文献中先前报道的那样，T管的有序横向排列在发育后期出现，早期的T-SR连接（三联体和二联体）沿T管随机分布，且主要呈纵向排列。我们发现，横管的最初出现相当突然，并且所有早期T管都呈纵向排列。T管网络的横向排列、三联体在A-I交界处的定位以及SR分化区域的发育是协调发生的事件，对于腿部肌肉而言，这些事件在大约3周的时间内逐渐发生。三联体发育的时间与报道的慢钙电流增加和二氢吡啶结合增加的时间一致。EDL和比目鱼肌纤维之间T管模式的差异仅在相对较晚的阶段才明显。
