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The expression of the bstVIM gene from Bacillus stearothermophilus V is restricted to vegetative cell growth.

作者信息

González E, Padilla C, Saavedra C, Vásquez C

机构信息

Laboratorio de Biología Molecular, Universidad de Talca, Chile.

出版信息

Microbiology (Reading). 1994 Jun;140 ( Pt 6):1337-40. doi: 10.1099/00221287-140-6-1337.

DOI:10.1099/00221287-140-6-1337
PMID:8081498
Abstract

The activity of BstVI DNA methyltransferase was monitored during the sporulative cycle of Bacillus stearothermophilus V. Significant methylase activity was found only in bacteria growing vegetatively. This was confirmed by Northern hybridization, which indicated that the bstVIM gene was not transcribed in cells undergoing sporulation. Supporting evidence came from experiments which demonstrated that the RNA polymerase holoenzyme from these cells did not recognize the promoter elements upstream of the bstVIM gene.

摘要

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