Karbanová Jana, Missol-Kolka Ewa, Fonseca Ana-Violeta, Lorra Christoph, Janich Peggy, Hollerová Hana, Jászai József, Ehrmann Jirí, Kolár Zdenek, Liebers Cornelia, Arl Stefanie, Subrtová Danuse, Freund Daniel, Mokry Jaroslav, Huttner Wieland B, Corbeil Denis
Department of Histology and Embryology, Faculty of Medicine in Hradec Králové, Charles University in Prague, Hradec Králové, Czech Republic.
J Histochem Cytochem. 2008 Nov;56(11):977-93. doi: 10.1369/jhc.2008.951897. Epub 2008 Jul 21.
Human prominin-1 (CD133) is expressed by various stem and progenitor cells originating from diverse sources. In addition to stem cells, its mouse ortholog is expressed in a broad range of adult epithelial cells, where it is selectively concentrated in their apical domain. The lack of detection of prominin-1 in adult human epithelia might be explained, at least in part, by the specificity of the widely used AC133 antibody, which recognizes an epitope that seems dependent on glycosylation. Here we decided to re-examine its expression in adult human tissues, particularly in glandular epithelia, using a novel monoclonal antibody (80B258) generated against the human prominin-1 polypeptide. In examined tissues, we observed 80B258 immunoreactivity at the apical or apicolateral membranes of polarized cells. For instance, we found expression in secretory serous and mucous cells as well as intercalated ducts of the large salivary and lacrimal glands. In sweat glands including the gland of Moll, 80B258 immunoreactivity was found in the secretory (eccrine and apocrine glands) and duct (eccrine glands) portion. In the liver, 80B258 immunoreactivity was identified in the canals of Hering, bile ductules, and small interlobular bile ducts. In the uterus, we detected 80B258 immunoreactivity in endometrial and cervical glands. Together these data show that the overall expression of human prominin-1 is beyond the rare primitive cells, and it seems to be a general marker of apical or apicolateral membrane of glandular epithelia. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
人类促红细胞生成素-1（CD133）由源自不同来源的各种干细胞和祖细胞表达。除干细胞外，其小鼠同源物在广泛的成年上皮细胞中表达，在这些细胞中它选择性地集中在其顶端区域。在成年人类上皮细胞中未检测到促红细胞生成素-1，这至少部分可以通过广泛使用的AC133抗体的特异性来解释，该抗体识别一个似乎依赖于糖基化的表位。在这里，我们决定使用针对人类促红细胞生成素-1多肽产生的新型单克隆抗体（80B258）重新检查其在成年人类组织中的表达，特别是在腺上皮中。在检查的组织中，我们在极化细胞的顶端或顶端外侧膜上观察到80B258免疫反应性。例如，我们在大唾液腺和泪腺的分泌浆液性和粘液性细胞以及闰管中发现了表达。在包括Moll腺的汗腺中，在分泌部（外分泌腺和顶泌腺）和导管部（外分泌腺）发现了80B258免疫反应性。在肝脏中，在赫林管、胆小管和小叶间小胆管中鉴定出80B258免疫反应性。在子宫中，我们在子宫内膜和宫颈腺中检测到80B258免疫反应性。这些数据共同表明，人类促红细胞生成素-1的整体表达超出了罕见的原始细胞，它似乎是腺上皮顶端或顶端外侧膜的一般标志物。本文稿包含在线补充材料，网址为http://www.jhc.org。请在线访问本文以查看这些材料。
