Shinya K, Awakura T, Shimada A, Silvano F D, Umemura T, Otsuki K
Departments of Veterinary Pathology and Public Health, Faculty of Agriculture, Tottori University, Tottori, Japan.
Avian Pathol. 1995 Dec;24(4):623-32. doi: 10.1080/03079459508419102.
Specific-pathogen-free (SPF), 2-day-old chicks were inoculated with type A influenza virus (A/whistling swan/Shimane/499/83/(H5N3)) into their caudal thoracic air sac. The original isolate of the virus was of low virulence (ICPI 0. 20 to 0.40), and was passaged 10 times through the respiratory organs of SPF chicks. Most of the chicks inoculated with the passaged virus (strain 499) showed respiratory and alimentary signs. Three of 30 chicks died on days 2, 6 and 7 post-inoculation (p.i.). Almost half of the infected chicks showed poor growth, and the variation of body size in the flock became prominent from day 10 p.i. Infected chicks consistently had pathological changes in the pancreas, liver, kidneys and respiratory tracts, and occasionally in the brain, duodenum and bone marrow. Positive immunoreaction to avian influenza virus (AIV) antigen and recovery of the virus persisted for longer period in the pancreas than in other organs. The pancreatic lesions were caused by a direct, lytic virus infection of the acinar cells and contributed to poor growth of the chicks.
将无特定病原体（SPF）的2日龄雏鸡通过尾侧胸气囊接种A型流感病毒（A/啸天鹅/岛根/499/83/（H5N3））。该病毒的原始分离株毒力较低（ICPI为0.20至0.40），并在SPF雏鸡的呼吸器官中传代10次。接种传代病毒（499株）的大多数雏鸡出现呼吸道和消化道症状。30只雏鸡中有3只在接种后第2、6和7天死亡。几乎一半的感染雏鸡生长不良，从接种后第10天起鸡群中个体大小差异变得明显。感染雏鸡的胰腺、肝脏、肾脏和呼吸道持续出现病理变化，偶尔在脑、十二指肠和骨髓中也有病变。对禽流感病毒（AIV）抗原的阳性免疫反应以及病毒的恢复在胰腺中持续的时间比在其他器官中更长。胰腺病变是由腺泡细胞的直接溶细胞性病毒感染引起的，导致雏鸡生长不良。
