Chen B Y, Hosi S, Nunoya T, Itakura C
Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan.
Avian Pathol. 1996 Jun;25(2):269-83. doi: 10.1080/03079459608419141.
Two-day-old specific-pathogen-free chicks were inoculated intranasally with the MA-87 strain of infectious bronchitis virus, and trachea and kidney lesions studied histologically and immunohistochemically. Lesions and viral antigen were first detected in the trachea; severe damage was then observed in the kidney. Viral antigen appeared prior to the development of renal lesions and was detected in the cytoplasm of epithelial cells by 4 days post-inoculation (p.i.). The epithelial cells of the collecting ducts, collecting tubules and distal convoluted tubules were first affected, followed by involvement of Henle's loops, whereas the proximal convoluted tubules were only minimally affected. Antigen-positive cells of ducts and tubules were degenerated and desquamated. The severe epithelial cell damage resulted in infiltration of heterophils and macrophages in the interstitium, ducts and tubules. The detection of viral antigen was consistent with the distribution of histological lesions at 6 to 8 days p.i. At a later stage, antigen-positive cells disappeared and repair of epithelial cells was seen, accompanied by interstitial lymphoplasmacytic infiltration and lymphoid nodular formation.
将两日龄的无特定病原体雏鸡经鼻内接种传染性支气管炎病毒MA - 87株，并对气管和肾脏病变进行组织学和免疫组织化学研究。病变和病毒抗原首先在气管中被检测到；随后在肾脏中观察到严重损伤。病毒抗原在肾脏病变出现之前就已出现，接种后4天（p.i.）在肾小管上皮细胞的细胞质中被检测到。集合管、集合小管和远曲小管的上皮细胞首先受到影响，随后亨氏袢也受到累及，而近曲小管仅受到轻微影响。导管和小管的抗原阳性细胞发生变性并脱落。严重的上皮细胞损伤导致间质、导管和小管中有嗜异性粒细胞和巨噬细胞浸润。接种后6至8天，病毒抗原的检测结果与组织学病变分布一致。在后期，抗原阳性细胞消失，可见上皮细胞修复，伴有间质淋巴细胞和浆细胞浸润以及淋巴小结形成。
