CSIRO Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
Avian Pathol. 1996 Dec;25(4):721-36. doi: 10.1080/03079459608419177.
A sandwich ELISA (sELISA) employing four monoclonal antibodies as capture and IgG from egg yolk of hyperimmune hens as detecting antibodies was developed for the simultaneous detection and differentiation of infectious bronchitis virus (IBV) antigen in both allantoic fluid and tissues from experimental and field infections. Between 10(2) and 10(3) median ciliostatic dose (CD50) of virus was required for the detection of the majority of IBV strains in allantoic fluid. In chicks infected with 10(4 )to 10(6) CD50 of virus there was a good agreement between sEOSA and virus isolation. EBV antigen was detected in trachea of chicks having virus titres of 10(2 )CD(50). Following vaccination with the recommended dose of two IB vaccines, IBV antigen was not detected by sELISA. There was also a good correlation between sELISA and virus isolation on samples obtained from field infections. In two broiler flocks with respiratory disease, IBV antigen was detected by sELISA in a high proportion of samples. In one flock, two antigenically different strains were detected, one of which was vaccine virus. The other IBV strain identified was a new antigenic variant not previously detected.
建立了一种夹心 ELISA（sELISA）方法，该方法使用 4 种单克隆抗体作为捕获抗体，以高免母鸡卵黄 IgG 作为检测抗体，可用于同时检测和区分实验感染和现场感染的鸡传染性支气管炎病毒（IBV）抗原在尿囊液和组织中的存在。在尿囊液中检测大多数 IBV 株时，需要 10(2)至 10(3)个半数细胞病变剂量（CD50）的病毒。在感染 10(4)至 10(6) CD50 病毒的鸡中，sEOSA 与病毒分离之间具有良好的一致性。在病毒滴度为 10(2) CD(50)的鸡的气管中检测到 EBV 抗原。用推荐剂量的两种 IB 疫苗进行免疫接种后，sELISA 未检测到 IBV 抗原。在田间感染样本中，sELISA 与病毒分离之间也存在良好的相关性。在有呼吸道疾病的两个肉鸡群中，sELISA 检测到高比例的样本中存在 IBV 抗原。在一个鸡群中，检测到了两种具有不同抗原性的毒株，其中一种是疫苗病毒。鉴定出的另一种 IBV 株是以前未检测到的新抗原变异株。
