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通过体外和鸡胚滴定法对传染性支气管炎冠状病毒进行定量分析。

Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo.

作者信息

Kint Joeri, Maier Helena Jane, Jagt Erik

机构信息

Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands,

出版信息

Methods Mol Biol. 2015;1282:89-98. doi: 10.1007/978-1-4939-2438-7_9.

DOI:10.1007/978-1-4939-2438-7_9
PMID:25720474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7121424/
Abstract

Quantification of the number of infectious viruses in a sample is a basic virological technique. In this chapter we provide a detailed description of three techniques to estimate the number of viable infectious avian coronaviruses in a sample. All three techniques are serial dilution assays, better known as titrations.

摘要

对样本中感染性病毒数量进行定量是一项基本的病毒学技术。在本章中,我们将详细描述三种用于估计样本中活的感染性禽冠状病毒数量的技术。这三种技术均为系列稀释测定法,更广为人知的名称是滴定法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/a9cb63931d2c/317916_1_En_9_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/afd0a00e6c58/317916_1_En_9_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/84908c54cbf5/317916_1_En_9_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/d68285467584/317916_1_En_9_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/a9cb63931d2c/317916_1_En_9_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/afd0a00e6c58/317916_1_En_9_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/84908c54cbf5/317916_1_En_9_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/d68285467584/317916_1_En_9_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669d/7121424/a9cb63931d2c/317916_1_En_9_Fig4_HTML.jpg

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2
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3
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4
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Front Vet Sci. 2023 Feb 1;10:1107059. doi: 10.3389/fvets.2023.1107059. eCollection 2023.
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