Giovannoli Cristina, Baggiani Claudio, Anfossi Laura, Giraudi Gianfranco
Department of Analytical Chemistry, University of Torino, Via Giuria 5, Torino, Italy.
Electrophoresis. 2008 Aug;29(16):3349-65. doi: 10.1002/elps.200800004.
Artificial biomimetic receptors, such as aptamers and molecular-imprinted polymers, show antibody-like properties which are due to molecular recognition phenomena characterized by high affinity and selectivity. These binding features have made them suitable in all those application fields in which selective recognition is required. Thus, it is not surprising that they are finding applications in affinity CE as well. Recently, a variety of ACE methods have shown themselves to be suitable tools to provide a detailed quantitative characterization of the thermodynamic and kinetic aspects of binding. At the same time, affinity CE can exploit the peculiarities of these binding interactions to set up CE-based analytical tools for the separation and the determination of specific target molecules in microscale formats. This review will provide a detailed description of affinity CE methods recently reported in the literature and related to these topics.
人工仿生受体，如适体和分子印迹聚合物，具有类似抗体的特性，这归因于以高亲和力和选择性为特征的分子识别现象。这些结合特性使其适用于所有需要选择性识别的应用领域。因此，它们在亲和毛细管电泳中也得到应用也就不足为奇了。最近，各种亲和毛细管电泳方法已证明自身是用于详细定量表征结合的热力学和动力学方面的合适工具。同时，亲和毛细管电泳可以利用这些结合相互作用的特性，建立基于毛细管电泳的分析工具，用于在微尺度形式下分离和测定特定目标分子。本综述将详细描述文献中最近报道的与这些主题相关的亲和毛细管电泳方法。
