Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin, P. R. China.
Int J Food Sci Nutr. 2012 May;63(3):338-42. doi: 10.3109/09637486.2011.627847. Epub 2011 Oct 21.
The thin-layer chromatographic analysis of the crude oligosaccharides extracted from yacon revealed the presence of glucose, fructose, sucrose, 1-kestose and nystose. The qualitative and quantitative analysis was carried out on oligosaccharides by high pressure liquid chromatography and the results showed that the contents of d-glucose, fructose, sucrose, 1-kestose, nystose and 1-fructofuranosyl nystose in oligosaccharides were 38.30%, 16.44%, 14.58%, 12.29%, 12.17%, 6.20%, respectively. The content of the fructooligosaccharides in oligosaccharides was 30.66%. The crude oligosaccharides were separated and purified by silica gel column chromatography. The two fractions obtained from crude oligosaccharides were 1-kestose and nystose, which were identified by mass spectra. The yield of 1-kestose and nystose were 10.36% and 9.73%, respectively. The purity of 1-kestose was 82.9% and of nystose was 73.6%.
从雪莲果中提取的粗低聚糖的薄层色谱分析表明，存在葡萄糖、果糖、蔗糖、1-蔗果三糖和棉子糖。采用高效液相色谱法对低聚糖进行了定性和定量分析，结果表明，低聚糖中 D-葡萄糖、果糖、蔗糖、1-蔗果三糖、棉子糖和 1-果糖基棉子糖的含量分别为 38.30%、16.44%、14.58%、12.29%、12.17%、6.20%。低聚糖中果聚糖的含量为 30.66%。粗低聚糖经硅胶柱色谱分离纯化。从粗低聚糖中得到的两个馏分是 1-蔗果三糖和棉子糖，通过质谱鉴定了这两种物质。1-蔗果三糖和棉子糖的收率分别为 10.36%和 9.73%。1-蔗果三糖的纯度为 82.9%，棉子糖的纯度为 73.6%。
