蔗果寡糖和水苏糖的色谱分析及雪莲果中 1-蔗果三糖和水苏糖的制备。
The chromatographic analysis of oligosaccharides and preparation of 1-kestose and nystose in yacon.
机构信息
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin, P. R. China.
出版信息
Int J Food Sci Nutr. 2012 May;63(3):338-42. doi: 10.3109/09637486.2011.627847. Epub 2011 Oct 21.
The thin-layer chromatographic analysis of the crude oligosaccharides extracted from yacon revealed the presence of glucose, fructose, sucrose, 1-kestose and nystose. The qualitative and quantitative analysis was carried out on oligosaccharides by high pressure liquid chromatography and the results showed that the contents of d-glucose, fructose, sucrose, 1-kestose, nystose and 1-fructofuranosyl nystose in oligosaccharides were 38.30%, 16.44%, 14.58%, 12.29%, 12.17%, 6.20%, respectively. The content of the fructooligosaccharides in oligosaccharides was 30.66%. The crude oligosaccharides were separated and purified by silica gel column chromatography. The two fractions obtained from crude oligosaccharides were 1-kestose and nystose, which were identified by mass spectra. The yield of 1-kestose and nystose were 10.36% and 9.73%, respectively. The purity of 1-kestose was 82.9% and of nystose was 73.6%.
从雪莲果中提取的粗低聚糖的薄层色谱分析表明，存在葡萄糖、果糖、蔗糖、1-蔗果三糖和棉子糖。采用高效液相色谱法对低聚糖进行了定性和定量分析，结果表明，低聚糖中 D-葡萄糖、果糖、蔗糖、1-蔗果三糖、棉子糖和 1-果糖基棉子糖的含量分别为 38.30%、16.44%、14.58%、12.29%、12.17%、6.20%。低聚糖中果聚糖的含量为 30.66%。粗低聚糖经硅胶柱色谱分离纯化。从粗低聚糖中得到的两个馏分是 1-蔗果三糖和棉子糖，通过质谱鉴定了这两种物质。1-蔗果三糖和棉子糖的收率分别为 10.36%和 9.73%。1-蔗果三糖的纯度为 82.9%，棉子糖的纯度为 73.6%。
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