Oliveira Pedro H, Lemos Francisco, Monteiro Gabriel A, Prazeres Duarte M F
IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, 1049-001 Lisbon, Portugal.
Plasmid. 2008 Sep;60(2):159-65. doi: 10.1016/j.plasmid.2008.06.004. Epub 2008 Aug 3.
In this study, a simple non-linear mathematical function is proposed to accurately predict recombination frequencies in bacterial plasmid DNA harbouring directly repeated sequences. The mathematical function, which was developed on the basis of published data on deletion-formation in multicopy plasmids containing direct-repeats (14-856 bp) and intervening sequences (0-3872 bp), also accounts for the strain genotype in terms of its recA function. A bootstrap resampling technique was used to estimate confidence intervals for the correlation parameters. More than 92% of the predicted values were found to be within a pre-established +/-5-fold interval of deviation from experimental data. The correlation does not only provide a way to predict, with good accuracy, the recombination frequency, but also opens the way to improve insight into these processes.
在本研究中，提出了一种简单的非线性数学函数，以准确预测含有直接重复序列的细菌质粒DNA中的重组频率。该数学函数是根据已发表的关于含有直接重复序列（14 - 856 bp）和间隔序列（0 - 3872 bp）的多拷贝质粒中缺失形成的数据开发的，同时也考虑了菌株基因型的recA功能。采用自助重采样技术来估计相关参数的置信区间。发现超过92%的预测值在预先设定的与实验数据偏差的±5倍区间内。这种相关性不仅提供了一种以良好准确性预测重组频率的方法，还为深入了解这些过程开辟了道路。
