Sümegi A, Birkó Z, Szeszák F, Vitális S, Biró S
Institute of Biology, University Medical School of Debrecen, Hungary.
Acta Biol Hung. 1997;48(3):275-9.
A spontaneous deletion probably caused by rec A independent recombination between short-direct repeat sequences was observed in pUC19 plasmid carrying a piece of Streptomyces genomic DNA after culturing in liquid medium. The deletion removed an unknown portion of the cloned DNA and the BamHI-EcoRI part of the multiple cloning site with an additional flanking 111 bp from the vector. At the junction a 13 bp GC-rich DNA sequence highly homologous to a known spontaneous deletion hotspot was found.
在携带链霉菌基因组DNA片段的pUC19质粒于液体培养基中培养后，观察到可能由短直接重复序列之间rec A非依赖性重组引起的自发缺失。该缺失去除了克隆DNA的未知部分以及多克隆位点的BamHI - EcoRI部分，并从载体额外移除了侧翼111 bp。在连接处发现了一个与已知自发缺失热点高度同源的13 bp富含GC的DNA序列。
