Dölken Gottfried, Dölken Lars, Hirt Carsten, Fusch Christoph, Rabkin Charles S, Schüler Frank
Department of Hematology and Oncology, Ernst-Moritz-Arndt-University Greifswald, Germany.
J Natl Cancer Inst Monogr. 2008(39):44-7. doi: 10.1093/jncimonographs/lgn005.
Circulating t(14;18)-positive cells were detected by quantitative real-time polymerase chain reaction on DNA isolated from peripheral blood mononuclear cells (PBMNCs) from 644 healthy individuals between <1 and 91 years of age. In all, 45% of all samples (287/644) were positive, and 40% of the positive samples (114/287) contained more than one positive clone. The prevalence of t(14;18)-positive cells showed a strong correlation with age. A total of 36 cord blood samples and 48 PBMNCs from children <10 years were negative. The prevalence of circulating positive cells increased from the second to fifth decade of life from 20% to 66% and remained stable thereafter. Also the median frequency of circulating t(14;18)-positive cells as well as the prevalence of multiple clones showed an increase with age. In all, 4% (24/644) of all blood samples contained >1 positive cell in 25,000 cells, a finding restricted to healthy individuals >40 years. These results are discussed in relation to the low incidence of follicular lymphoma.
通过定量实时聚合酶链反应，对644名年龄在1岁至91岁之间的健康个体外周血单个核细胞（PBMNC）中分离出的DNA进行检测，以检测循环中的t(14;18)阳性细胞。总体而言，所有样本中有45%（287/644）呈阳性，且40%的阳性样本（114/287）含有不止一个阳性克隆。t(14;18)阳性细胞的患病率与年龄呈强相关。来自10岁以下儿童的36份脐带血样本和48份PBMNC均为阴性。循环阳性细胞的患病率从生命的第二个十年到第五个十年从20%增加到66%，此后保持稳定。此外，循环t(14;18)阳性细胞的中位频率以及多个克隆的患病率也随年龄增加。总体而言，所有血样中有4%（24/644）在25,000个细胞中含有>1个阳性细胞，这一发现仅限于40岁以上的健康个体。结合滤泡性淋巴瘤的低发病率对这些结果进行了讨论。
