Schirmer Christina, Meisel Hans
Department of Safety and Quality of Milk and Fish Products, Max Rubner Institute, Federal Research Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, 24103, Kiel, Germany.
Anal Bioanal Chem. 2008 Sep;392(1-2):223-9. doi: 10.1007/s00216-008-2269-4. Epub 2008 Jul 22.
A variety of bulk polymers for the selective separation of chloramphenicol were synthesised from 2-vinylpyridine, diethylaminoethyl methacrylate or methacrylic acid monomers. Chromatographic evaluation indicated that chloramphenicol was retained under nonpolar elution conditions (k = 58.65) through selective hydrogen bonding and ionic interactions. The retention of chloramphenicol under aqueous elution conditions (k > 100) results from nonselective hydrophobic interactions. Under nonpolar elution conditions, the functional monomer employed imparted a significant influence on the recognition properties of the corresponding polymer. After solid-phase extraction using a molecularly imprinted polymer as sorbent and either an organic or aqueous washing solvent, nearly 100% recovery from the chloramphenicol standard solution was achieved, and nearly 90% recovery could be attained from spiked honey samples. The molecularly imprinted polymer was well suited to suppress matrix effects, and provided optimal preconcentration of the target molecule (chloramphenicol) prior to chromatographic analysis.
由2-乙烯基吡啶、甲基丙烯酸二乙氨基乙酯或甲基丙烯酸单体合成了多种用于选择性分离氯霉素的本体聚合物。色谱评估表明，氯霉素在非极性洗脱条件下（k = 58.65）通过选择性氢键和离子相互作用被保留。氯霉素在水相洗脱条件下（k > 100）的保留是由非选择性疏水相互作用导致的。在非极性洗脱条件下，所用功能单体对相应聚合物的识别性能有显著影响。使用分子印迹聚合物作为吸附剂并采用有机或水相洗涤溶剂进行固相萃取后，从氯霉素标准溶液中实现了近100%的回收率，从加标蜂蜜样品中可实现近90%的回收率。该分子印迹聚合物非常适合抑制基质效应，并在色谱分析之前对目标分子（氯霉素）进行了最佳预富集。
