Marine College, Shandong University, Weihai 264209, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Mar Pollut Bull. 2018 Aug;133:750-755. doi: 10.1016/j.marpolbul.2018.06.041. Epub 2018 Jun 22.
This study highlights an efficient sample pre-treatment method for preconcentration and detection of chloramphenicol in marine water using molecularly imprinted solid-phase extraction (MISPE). Chloramphenicol molecularly imprinted microspheres were prepared and evaluated on the base of morphology, capacity and selectivity. The imprinted microspheres exhibited specific recognition and high retention capability to chloramphenicol and were applied as special solid-phase extraction adsorbents. An off-line MISPE protocol has been optimized and a creative analytical method coupled to HPLC-DAD was successfully developed for the cleanup and determination of chloramphenicol in seawater samples. Method performance was satisfactory with recoveries ranging from 81 to 90% and relative standard deviation (RSD) was <4.93% (n = 3). Accuracy of the method was assessed at three spiking concentration levels and the limit of detection was 5 ng L. Finally, five seawater samples from Jiaozhou Bay of China were determined and the results showed that there was no chloramphenicol detected.
本研究采用分子印迹固相萃取（MISPE），提出了一种高效的海水样品中氯霉素的预浓缩和检测前处理方法。基于形态、容量和选择性，制备并评价了氯霉素分子印迹微球。印迹微球对氯霉素表现出特异性识别和高保留能力，可作为特殊固相萃取吸附剂使用。优化了离线 MISPE 方案，并成功开发了一种与 HPLC-DAD 耦合的创新分析方法，用于海水样品中氯霉素的净化和测定。方法性能令人满意，回收率在 81%至 90%之间，相对标准偏差（RSD）小于 4.93%（n=3）。在三个加标浓度水平评估了方法的准确性，检测限为 5ng/L。最后，测定了来自中国胶州湾的五个海水样本，结果表明未检测到氯霉素。
