Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Food Chem. 2013 Nov 1;141(1):23-8. doi: 10.1016/j.foodchem.2013.02.085. Epub 2013 Mar 7.
A rapid and selective method was successfully developed using magnetic molecularly imprinted polymer (MMIP) as sorbent for the extraction of chloramphenicol from honey samples. The extraction process was carried out in a single step by blending and stirring the sample, extraction solvent and polymers. When the extraction was completed, the MMIPs adsorbing the analyte were separated from the sample matrix by an external magnet. The analyte eluted from the MMIPs by methanol under ultrasound assisted was analysed by liquid chromatography-tandem mass spectrometry. Various parameters affecting the extraction efficiency were evaluated for achieving optimal recovery and reducing non-specific interactions. Under optimal conditions, the detection limit of CAP was 0.047 ng g(-1). The relative standard deviations of intra- and inter-day ranging from 4.1% to 5.3% and from 2.9% to 7.1% were obtained, respectively. The method was applied to determine CAP in six honey samples. The recoveries of CAP in these samples from 84.3% to 90.9% were obtained.
成功开发了一种快速、选择性的方法，使用磁性分子印迹聚合物 (MMIP) 作为吸附剂从蜂蜜样品中提取氯霉素。萃取过程通过混合和搅拌样品、萃取溶剂和聚合物一步完成。萃取完成后，通过外部磁铁将吸附分析物的 MMIP 从样品基质中分离出来。用甲醇在超声辅助下从 MMIP 中洗脱分析物，并用液相色谱-串联质谱法进行分析。评估了各种影响萃取效率的参数，以实现最佳的回收率并减少非特异性相互作用。在最佳条件下，CAP 的检测限为 0.047 ng g(-1)。获得的内标和日间相对标准偏差分别为 4.1%至 5.3%和 2.9%至 7.1%。该方法用于测定六种蜂蜜样品中的 CAP。从这些样品中 CAP 的回收率为 84.3%至 90.9%。
