Liu Hong Ling, Sonn Chung Hee, Wu Jun Hua, Lee Kyung-Mi, Kim Young Keun
Research Institute of Engineering and Technology, Korea University, Seoul 136-713, Republic of Korea.
Biomaterials. 2008 Oct;29(29):4003-11. doi: 10.1016/j.biomaterials.2008.06.031. Epub 2008 Jul 22.
We explored the feasibility of magnetite (Fe(3)O(4))-gold (Au) core-shell nanocrystals as a useful vehicle for biomedical application such as cell separation. Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe(3)O(4)-Au core-shell nanocrystals using a carbodimide activation protocol. These nanocrystals were further tested for their ability to bind CD4+ T lymphocytes, bound to biotin-labeled anti-CD4 mAbs, isolated from the spleen of C57BL/6 mice. Our data show that the Fe(3)O(4)-Au nanocrystals successfully pulled down CD4+ T lymphocytes from the whole splenocytes with high specificity. Therefore, our nanocrystals provide an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application including diagnosis, monitoring, and treatment of human diseases.
我们探索了磁铁矿（Fe(3)O(4)）-金（Au）核壳纳米晶体作为细胞分离等生物医学应用有用载体的可行性。使用碳二亚胺活化方案将链霉亲和素-异硫氰酸荧光素（STA-FITC）偶联到Fe(3)O(4)-Au核壳纳米晶体的表面。对这些纳米晶体结合从C57BL/6小鼠脾脏分离的、与生物素标记的抗CD4单克隆抗体结合的CD4+ T淋巴细胞的能力进行了进一步测试。我们的数据表明，Fe(3)O(4)-Au纳米晶体成功地以高特异性从全脾细胞中分离出CD4+ T淋巴细胞。因此，我们的纳米晶体为细胞分离过程提供了一种有效工具，并进一步展现出应用于包括人类疾病诊断、监测和治疗在内的其他生物医学应用领域的巨大潜力。
