• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于FeO-Au核壳纳米颗粒多种抑制剂的细胞摄取效率评估：控制大肠癌细胞特异性内吞作用的可能性

Assessment of Cellular Uptake Efficiency According to Multiple Inhibitors of FeO-Au Core-Shell Nanoparticles: Possibility to Control Specific Endocytosis in Colorectal Cancer Cells.

作者信息

Park Bo Gi, Kim Yu Jin, Min Ji Hyun, Cheong Taek-Chin, Nam Sang Hwan, Cho Nam-Hyuk, Kim Young Keun, Lee Kyu Back

机构信息

Department of Biomedical Engineering, College of Health Science, Korea University, Seoul, 02841, South Korea.

Institute for High Technology Materials and Devices, College of Engineering, Korea University, Seoul, 02841, South Korea.

出版信息

Nanoscale Res Lett. 2020 Aug 17;15(1):165. doi: 10.1186/s11671-020-03395-w.

DOI:10.1186/s11671-020-03395-w
PMID:32804261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7431494/
Abstract

Magnetite (FeO)-gold (Au) core-shell nanoparticles (NPs) have unique magnetic and optical properties. When combined with biological moieties, these NPs can offer new strategies for biomedical applications, such as drug delivery and cancer targeting. Here, we present an effective method for the controllable cellular uptake of magnetic core-shell NP systems combined with biological moieties. Vimentin, which is the structural protein, has been biochemically confirmed to affect phagocytosis potently. In addition, vimentin affects exogenic materials internalization into cells even though under multiple inhibitions of biological moieties. In this study, we demonstrate the cellular internalization performance of FeO-Au core-shell NPs with surface modification using a combination of biological moieties. The photofluorescence of vimentin-tagged NPs remained unaffected under multiple inhibition tests, indicating that the NPs were minimally influenced by nystatin, dynasore, cytochalasin D, and even the Muc1 antibody (Ab). Consequently, this result indicates that the Muc1 Ab can target specific molecules and can control specific endocytosis. Besides, we show the possibility of controlling specific endocytosis in colorectal cancer cells.

摘要

磁铁矿（FeO）-金（Au）核壳纳米颗粒（NPs）具有独特的磁性和光学特性。当与生物部分结合时，这些纳米颗粒可为生物医学应用提供新策略，如药物递送和癌症靶向。在此，我们提出一种有效方法，用于可控地将磁性核壳NP系统与生物部分结合并使其被细胞摄取。波形蛋白作为一种结构蛋白，已通过生物化学方法证实其能有效影响吞噬作用。此外，波形蛋白即使在生物部分的多种抑制作用下，仍会影响外源性物质进入细胞。在本研究中，我们展示了通过结合生物部分对表面进行修饰的FeO-Au核壳纳米颗粒的细胞内化性能。在多次抑制试验下，波形蛋白标记的纳米颗粒的光荧光保持不变，这表明纳米颗粒受制霉菌素、dynasore、细胞松弛素D甚至Muc1抗体（Ab）的影响极小。因此，该结果表明Muc1抗体可靶向特定分子并能控制特定的内吞作用。此外，我们展示了在结肠癌细胞中控制特定内吞作用的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/871e1108946b/11671_2020_3395_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/b82ad055256e/11671_2020_3395_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/0b225e259fac/11671_2020_3395_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/7620ff30c564/11671_2020_3395_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/84430eef8856/11671_2020_3395_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/bc66015671a7/11671_2020_3395_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/871e1108946b/11671_2020_3395_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/b82ad055256e/11671_2020_3395_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/0b225e259fac/11671_2020_3395_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/7620ff30c564/11671_2020_3395_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/84430eef8856/11671_2020_3395_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/bc66015671a7/11671_2020_3395_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ee2/7431494/871e1108946b/11671_2020_3395_Fig6_HTML.jpg

相似文献

1
Assessment of Cellular Uptake Efficiency According to Multiple Inhibitors of FeO-Au Core-Shell Nanoparticles: Possibility to Control Specific Endocytosis in Colorectal Cancer Cells.基于FeO-Au核壳纳米颗粒多种抑制剂的细胞摄取效率评估：控制大肠癌细胞特异性内吞作用的可能性
Nanoscale Res Lett. 2020 Aug 17;15(1):165. doi: 10.1186/s11671-020-03395-w.
2
Multi-functional core-shell FeO@Au nanoparticles for cancer diagnosis and therapy.多功能核壳型 FeO@Au 纳米粒子用于癌症的诊断与治疗。
Colloids Surf B Biointerfaces. 2019 Feb 1;174:252-259. doi: 10.1016/j.colsurfb.2018.11.004. Epub 2018 Nov 15.
3
Magnetic/NIR-responsive drug carrier, multicolor cell imaging, and enhanced photothermal therapy of gold capped magnetite-fluorescent carbon hybrid nanoparticles.金包覆磁-荧光碳杂化纳米粒子的磁/近红外响应药物载体、多色细胞成像及光热治疗增强作用。
Nanoscale. 2015 May 7;7(17):7885-95. doi: 10.1039/c4nr07335e.
4
Green fabrication of biologically active magnetic core-shell FeO/Au nanoparticles and their potential anticancer effect.绿色合成生物活性磁性核壳 FeO/Au 纳米粒子及其潜在的抗癌作用。
Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:51-57. doi: 10.1016/j.msec.2018.11.008. Epub 2018 Nov 6.
5
Fabrication and spectroscopic studies of folic acid-conjugated Fe3O4@Au core-shell for targeted drug delivery application.用于靶向给药应用的叶酸共轭Fe3O4@Au核壳结构的制备及光谱研究
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Sep 5;148:146-55. doi: 10.1016/j.saa.2015.03.078. Epub 2015 Mar 26.
6
The role of morphology, shell composition and protein corona formation in Au/FeO composite nanoparticle mediated macrophage responses.形态、壳组成和蛋白冠形成在 Au/FeO 复合纳米颗粒介导的巨噬细胞反应中的作用。
J Mater Chem B. 2021 Aug 28;9(32):6387-6395. doi: 10.1039/d1tb01026c. Epub 2021 Jul 26.
7
Preparation of fluorescent Au-SiO core-shell nanoparticles and nanorods with tunable silica shell thickness and surface modification for immunotargeting.具有可调硅壳厚度和用于免疫靶向的表面修饰的荧光金-二氧化硅核壳纳米颗粒和纳米棒的制备。
J Mater Chem B. 2016 Aug 28;4(32):5418-5428. doi: 10.1039/c6tb01659f. Epub 2016 Jul 27.
8
Synthesis, characterization and biomedical application of multifunctional luminomagnetic core-shell nanoparticles.多功能发光磁性核壳纳米粒子的合成、表征及生物医学应用。
Mater Sci Eng C Mater Biol Appl. 2015 Jan;46:32-40. doi: 10.1016/j.msec.2014.10.002. Epub 2014 Oct 9.
9
pH sensitive core-shell magnetic nanoparticles for targeted drug delivery in cancer therapy.用于癌症治疗中靶向药物递送的pH敏感核壳磁性纳米粒子。
Rom J Morphol Embryol. 2016;57(1):23-32.
10
Size tunable Au@Ag core-shell nanoparticles: synthesis and surface-enhanced Raman scattering properties.尺寸可调的 Au@Ag 核壳纳米粒子：合成与表面增强拉曼散射性质。
Langmuir. 2013 Dec 3;29(48):15076-82. doi: 10.1021/la403707j. Epub 2013 Nov 21.

引用本文的文献

1
Shape-dependent cellular uptake of iron oxide nanorods: mechanisms of endocytosis and implications on cell labeling and cellular delivery.形态依赖性的氧化铁纳米棒细胞摄取：内吞作用的机制及其对细胞标记和细胞递送的影响。
Nanoscale. 2024 Nov 28;16(46):21398-21415. doi: 10.1039/d4nr02408g.
2
Spermine-Based Poly(β-amino ester)s for siRNA Delivery against Mutated KRAS in Lung Cancer.基于精胺的聚（β-氨基酯）用于递送针对肺癌中突变型 KRAS 的 siRNA。
Mol Pharm. 2023 Sep 4;20(9):4505-4516. doi: 10.1021/acs.molpharmaceut.3c00206. Epub 2023 Aug 14.
3
Graphene Oxide Enhances Biogenesis and Release of Exosomes in Human Ovarian Cancer Cells.

本文引用的文献

1
Magnetic nanoarchitectures for cancer sensing, imaging and therapy.用于癌症检测、成像和治疗的磁性纳米结构
J Mater Chem B. 2019 Jan 7;7(1):9-23. doi: 10.1039/c8tb02741b. Epub 2018 Dec 11.
2
Improving cancer immunotherapy through nanotechnology.通过纳米技术提高癌症免疫疗法。
Nat Rev Cancer. 2019 Oct;19(10):587-602. doi: 10.1038/s41568-019-0186-9. Epub 2019 Sep 6.
3
Delivery technologies for cancer immunotherapy.癌症免疫疗法的递药技术。
氧化石墨烯增强人卵巢癌细胞外泌体的生物发生和释放。
Int J Nanomedicine. 2022 Nov 28;17:5697-5731. doi: 10.2147/IJN.S385113. eCollection 2022.
4
Using BioPAX-Parser (BiP) to enrich lists of genes or proteins with pathway data.使用 BioPAX-Parser（BiP）为基因或蛋白质列表富集途径数据。
BMC Bioinformatics. 2021 Sep 30;22(Suppl 13):376. doi: 10.1186/s12859-021-04297-z.
Nat Rev Drug Discov. 2019 Mar;18(3):175-196. doi: 10.1038/s41573-018-0006-z.
4
Double domain polyethylenimine-based nanoparticles for integrin receptor mediated delivery of plasmid DNA.基于双域聚亚乙基亚胺的纳米颗粒用于整合素受体介导的质粒 DNA 递释。
Sci Rep. 2018 May 1;8(1):6842. doi: 10.1038/s41598-018-25277-z.
5
Magnetic separation: its application in mining, waste purification, medicine, biochemistry and chemistry.磁分离：在采矿、废物净化、医药、生物化学和化学中的应用。
Chem Soc Rev. 2017 Oct 2;46(19):5925-5934. doi: 10.1039/c7cs00230k.
6
Targeted delivery system for cancer cells consist of multiple ligands conjugated genetically modified CCMV capsid on doxorubicin GNPs complex.针对癌细胞的靶向投递系统包含多个配体，这些配体通过基因修饰的 CCMV 衣壳与阿霉素 GNPs 复合物相连。
Sci Rep. 2016 Nov 22;6:37096. doi: 10.1038/srep37096.
7
Au@MnS@ZnS Core/Shell/Shell Nanoparticles for Magnetic Resonance Imaging and Enhanced Cancer Radiation Therapy.用于磁共振成像和增强癌症放射治疗的金@硫化锰@硫化锌核/壳/壳纳米颗粒
ACS Appl Mater Interfaces. 2016 Apr 20;8(15):9557-64. doi: 10.1021/acsami.5b11588. Epub 2016 Apr 7.
8
Iron Oxide Based Nanoparticles for Multimodal Imaging and Magnetoresponsive Therapy.用于多模态成像和磁响应治疗的氧化铁基纳米颗粒
Chem Rev. 2015 Oct 14;115(19):10637-89. doi: 10.1021/acs.chemrev.5b00112. Epub 2015 Aug 7.
9
The role of ROS generation from magnetic nanoparticles in an alternating magnetic field on cytotoxicity.交变磁场中磁性纳米颗粒产生的活性氧在细胞毒性方面的作用。
Acta Biomater. 2015 Oct;25:284-90. doi: 10.1016/j.actbio.2015.06.037. Epub 2015 Jul 2.
10
Magnetic nanoparticle-based hyperthermia for cancer treatment.基于磁性纳米颗粒的热疗用于癌症治疗。
Rep Pract Oncol Radiother. 2013 Nov 1;18(6):397-400. doi: 10.1016/j.rpor.2013.09.011.