猫多聚糖体的凝集素组织化学

Lectin histochemistry of feline polyglucosan bodies.

作者信息

Kamiya S, Suzuki Y, Yamano S, Daigo M

机构信息

Department of Veterinary Anatomy, Nippon Veterinary and Zootechnical College, Tokyo, Japan.

出版信息

J Comp Pathol. 1991 Feb;104(2):141-5. doi: 10.1016/s0021-9975(08)80097-7.

DOI:10.1016/s0021-9975(08)80097-7

PMID:1865024

Abstract

Lectin histochemistry was conducted to identify sugar residues of the polyglucosan bodies (PGB) in the feline brain. PGB stained with Concanavalia ensiformis agglutinin (Con A) and peanut agglutinin, but not with soybean agglutinin, wheat germ agglutinin, Dolichos biflorus agglutinin, Ricinus communis agglutinin or Ulex europaeus agglutinin. In addition, PGB bound to Con A after periodate oxidation. These results suggest that feline PGB contain mannose and galactose in addition to glucose and that the partial component of PGB is derived from rough endoplasmic reticulum and the Golgi apparatus.

摘要

采用凝集素组织化学方法鉴定猫脑内多聚葡萄糖体（PGB）的糖残基。PGB 与刀豆球蛋白 A（Con A）和花生凝集素染色阳性，但与大豆凝集素、麦胚凝集素、双花扁豆凝集素、蓖麻凝集素或荆豆凝集素染色阴性。此外，高碘酸盐氧化后 PGB 与 Con A 结合。这些结果表明，猫的 PGB 除含有葡萄糖外，还含有甘露糖和半乳糖，且 PGB 的部分成分来源于粗面内质网和高尔基体。