Alroy J, Goyal V, Skutelsky E
Histochemistry. 1987;86(6):603-7. doi: 10.1007/BF00489554.
Lectin-histochemical studies were performed on formalin-fixed, paraffin-embedded tissues from ten mammalian species to demonstrate the pattern of carbohydrate residues in vascular endothelium. Ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (ABC) was used as visualant. Ricinus communis agglutinin-I (RCA-I) and wheat germ agglutinin (WGA) stained vascular endothelium in all species. Peanut agglutinin (PNA) stained vascular endothelium in all species only after preincubation with neuraminidase. Bandeirea simplicifolia agglutinin-I (BS-I) stained vascular endothelium in all species but human, while Ulex europeus agglutinin-I (UEA-I) stained only human endothelium. Individual differences in staining of human vascular endothelium were noted with BS-I and succinylated-WGA (SWGA). Similarly, individual differences in staining of animal vascular endothelium were noted with soybean agglutinin (SBA) after preincubation with neuraminidase. Finally, Concanavalia ensiformis agglutinin (Con A), Dolichos biflorus agglutinin (DBA) and Lens culinaris agglutinin (LCA) did not stain vascular endothelium in any of the species studied.
对来自十种哺乳动物的福尔马林固定、石蜡包埋组织进行了凝集素组织化学研究，以展示血管内皮中碳水化合物残基的模式。使用十种不同的生物素化凝集素作为探针，并使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）作为可视化剂。蓖麻凝集素-I（RCA-I）和麦胚凝集素（WGA）在所有物种中均能染色血管内皮。花生凝集素（PNA）仅在与神经氨酸酶预孵育后才能在所有物种中染色血管内皮。单叶豆凝集素-I（BS-I）能在除人类以外的所有物种中染色血管内皮，而欧洲荆豆凝集素-I（UEA-I）仅能染色人类内皮。用BS-I和琥珀酰化WGA（SWGA）观察到人类血管内皮染色存在个体差异。同样，用神经氨酸酶预孵育后，用大豆凝集素（SBA）观察到动物血管内皮染色存在个体差异。最后，刀豆球蛋白A（Con A）、双花扁豆凝集素（DBA）和菜豆凝集素（LCA）在所研究的任何物种中均未染色血管内皮。
