Nielsen Anders R, Erikstrup Christian, Johansen Julia S, Fischer Christian P, Plomgaard Peter, Krogh-Madsen Rikke, Taudorf Sarah, Lindegaard Birgitte, Pedersen Bente K
Department of InfectiousDiseases, Facultyof Health Sciences, Centre of Inflammation and Metabolism, Copenhagen Muscle Research Center, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Diabetes. 2008 Nov;57(11):3078-82. doi: 10.2337/db08-0182. Epub 2008 Jul 23.
YKL-40 is produced by macrophages, and plasma YKL-40 is elevated in patients with diseases characterized by inflammation. In the present study, YKL-40 was examined in relation to obesity, inflammation, and type 2 diabetes.
Plasma YKL-40 and adipose tissue YKL-40 mRNA levels were investigated in 199 subjects who were divided into four groups depending on the presence or absence of type 2 diabetes and obesity. In addition, plasma YKL-40 was examined in healthy subjects during a hyperglycemic clamp, in which the plasma glucose level was kept at 15 mmol/l for 3 h, and during a hyperinsulinemic-euglycemic clamp.
Patients with type 2 diabetes had higher plasma YKL-40 (76.7 vs. 45.1 ng/ml, P = 0.0001) but not higher expression in adipose tissue YKL-40 mRNA (1.20 vs. 0.98, P = 0.2) compared with subjects with a normal glucose tolerance. Within the groups with normal glucose tolerance and type 2 diabetes, obesity subgroups showed no difference with respect to either plasma YKL-40 or adipose tissue YKL-40 mRNA levels. Multivariate regression analysis showed that plasma YKL-40 was associated with fasting plasma glucose (beta = 0.5, P = 0.0014) and plasma interleukin (IL)-6 (beta = 0.2, P = 0.0303). Plasma YKL-40 was not related to parameters of obesity. There were no changes in plasma YKL-40 in healthy subjects during either hyperglycemic or hyperinsulinemic-euglycemic clamps.
Plasma YKL-40 was identified as an obesity-independent marker of type 2 diabetes related to fasting plasma glucose and plasma IL-6 levels.
YKL - 40由巨噬细胞产生，在以炎症为特征的疾病患者中血浆YKL - 40水平升高。在本研究中，对YKL - 40与肥胖、炎症和2型糖尿病的关系进行了检测。
对199名受试者的血浆YKL - 40和脂肪组织YKL - 40 mRNA水平进行了研究，这些受试者根据是否患有2型糖尿病和肥胖被分为四组。此外，在健康受试者进行高血糖钳夹（在此期间血浆葡萄糖水平维持在15 mmol/l 3小时）和高胰岛素 - 正常血糖钳夹期间检测血浆YKL - 40。
与糖耐量正常的受试者相比，2型糖尿病患者的血浆YKL - 40水平更高（76.7对45.1 ng/ml，P = 0.0001），但脂肪组织YKL - 40 mRNA的表达无显著升高（1.20对0.98，P = 0.2）。在糖耐量正常和2型糖尿病组中，肥胖亚组在血浆YKL - 40或脂肪组织YKL - 40 mRNA水平方面无差异。多变量回归分析显示，血浆YKL - 40与空腹血糖（β = 0.5，P = 0.0014）和血浆白细胞介素（IL）-6（β = 0.2，P = 0.0303）相关。血浆YKL - 40与肥胖参数无关。在高血糖或高胰岛素 - 正常血糖钳夹期间，健康受试者的血浆YKL - 40无变化。
血浆YKL - 40被确定为一种与空腹血糖和血浆IL - 6水平相关的、不依赖于肥胖的2型糖尿病标志物。
