Fooladi Abbas Ali Imani, Sattari Morteza, Hassan Zuhair Mohammad, Mahdavi Mehdi, Azizi Taghi, Horii Akira
Research Center of Molecular Biology and Department of Microbiology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Biotechnol Lett. 2008 Dec;30(12):2053-9. doi: 10.1007/s10529-008-9805-3. Epub 2008 Jul 24.
The bacterial superantigen staphylococcal enterotoxin B (SEB) is a potent inducer of cytotoxic T-cell activity and cytokine production in vivo. We investigated the possibility of the therapeutic application of SEB in patients with fibrosarcoma. The anti-tumor effect of SEB in mice with inoculated fibrosarcoma (WEHI-164) was examined by intravenous (IV) and intratumoral (IT) injection and the sizes of the inoculated tumors, IFN-gamma production, and CD4+/CD8+ T cell infiltration were determined. The inoculated tumors were also examined histologically. In the mice in the IV-injected group, a significant reduction (P < 0.02) of tumor size was observed in comparison with mice in the IT-injected and control groups. Furthermore, the mice in the IV-injected group showed significantly higher levels of IFN-gamma (P < 0.009) and CD4+/CD8+ T cell infiltration when compared with the other groups (P < 0.02). A significantly higher frequency of necrosis in tumor tissues was also observed in mice in the IV-injected group (P < 0.05). Our present findings suggest that tumor cell death is caused by increased cytotoxic T-cell activity and cytokine levels in response to the IV injection of SEB and that SEB may be a good option for use as a novel therapy in patients with fibrosarcoma.
细菌超抗原葡萄球菌肠毒素B（SEB）是体内细胞毒性T细胞活性和细胞因子产生的强效诱导剂。我们研究了SEB在纤维肉瘤患者中治疗应用的可能性。通过静脉内（IV）和瘤内（IT）注射检查SEB对接种纤维肉瘤（WEHI-164）小鼠的抗肿瘤作用，并测定接种肿瘤的大小、IFN-γ产生以及CD4+/CD8+ T细胞浸润情况。还对接种肿瘤进行了组织学检查。在静脉注射组的小鼠中，与瘤内注射组和对照组的小鼠相比，观察到肿瘤大小显著减小（P < 0.02）。此外，与其他组相比，静脉注射组的小鼠显示出显著更高水平的IFN-γ（P < 0.009）和CD4+/CD8+ T细胞浸润（P < 0.02）。在静脉注射组的小鼠中还观察到肿瘤组织中坏死频率显著更高（P < 0.05）。我们目前的研究结果表明，静脉注射SEB后，细胞毒性T细胞活性和细胞因子水平升高导致肿瘤细胞死亡，并且SEB可能是纤维肉瘤患者新型治疗的良好选择。
