Li Fei, Yang Xiu-Wei
State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
Biomed Chromatogr. 2008 Nov;22(11):1206-12. doi: 10.1002/bmc.1046.
Dehydrodiisoeugenol (DDIE) is a bioactive neolignan from the seeds of Myristica fragrans Houtt., which exhibits good anti-inflammatory activity. A rapid and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of DDIE in rat tissues after intravenous administration. The tissue samples were processed by liquid-liquid extraction. The analyses were successfully carried out on a Diamonsiltrade mark ODS C18 column (250 x 4.6 mm i.d., 5 microm) equipped with a C18 guard column (8 x 4.6 mm i.d., 5 microm). The mobile phase was the system of methanol-water (4:1, v/v). The UV detection was set at 270 nm. The calibration curves were linear from 0.4 to 200.0 microg/g with the correlation coefficients (r(2)) greater than 0.998. The intra- and inter-day precisions in quality control samples were less than 10% and the accuracies were in the range 85.4-110.3%. The average recoveries from all the tissues were between 84.4 and 106.0%. This assay method has been successfully used to study the tissues distribution of DDIE in rats after intravenous administration. The result suggests that DDIE is distributed to rat tissues rapidly with possibly greater initial concentrations in liver and brain than in other tissues.
脱氢二异丁香酚（DDIE）是一种从肉豆蔻种子中提取的具有生物活性的新木脂素，具有良好的抗炎活性。已开发出一种快速简便的高效液相色谱（HPLC）方法，用于测定大鼠静脉给药后组织中的DDIE。组织样品通过液液萃取进行处理。分析在配备C18保护柱（8×4.6 mm内径，5μm）的Diamonsil商标ODS C18柱（250×4.6 mm内径，5μm）上成功进行。流动相为甲醇 - 水体系（4:1，v/v）。紫外检测波长设定为270 nm。校准曲线在0.4至200.0μg/g范围内呈线性，相关系数（r²）大于0.998。质量控制样品的日内和日间精密度小于10%，准确度在85.4 - 110.3%范围内。所有组织的平均回收率在84.4%至106.0%之间。该测定方法已成功用于研究大鼠静脉给药后DDIE的组织分布情况。结果表明，DDIE在大鼠组织中分布迅速，肝脏和大脑中的初始浓度可能高于其他组织。
