Li Fei, Yang Xiu-Wei
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, People's Republic of China.
Planta Med. 2008 Jun;74(8):880-4. doi: 10.1055/s-2008-1074545. Epub 2008 Jun 3.
A rapid and simple high-performance liquid chromatographic (HPLC) method was developed and validated to simultaneously analyze the diastereomers of (+)-licarin A and isolicarin A in rat plasma after intravenous administration. The analytes were extracted from the plasma by solid-phase extraction (SPE). Diastereomeric separation and determination were successfully achieved using a Diamonsil ODS C (18) reversed-phase column (250 mm x 4.6 mm i. d., 5 microm) with an RP18 guard column (8 mm x 4.6 mm i. d., 5 microm) and a mobile phase of MeOH-H (2)O (4 : 1, v/v). UV detection was at 270 nm. The linear ranges of the standard curves were 0.25 - 150.00 microg/mL for (+)-licarin A and 0.10 - 75.00 microg/mL for isolicarin A. The lower limits of detection and quantification were 0.05 and 0.25 microg/mL for (+)-licarin A, and 0.05 and 0.10 microg/mL for isolicarin A, respectively. This assay method was successfully applied to study the pharmacokinetics of diastereomers (+)-licarin A and isolicarin A in rat plasma.
建立并验证了一种快速简便的高效液相色谱（HPLC）方法，用于同时分析大鼠静脉注射后血浆中（+）-里卡林A和异里卡林A的非对映异构体。通过固相萃取（SPE）从血浆中提取分析物。使用Diamonsil ODS C（18）反相柱（250 mm×4.6 mm内径，5μm）和RP18保护柱（8 mm×4.6 mm内径，5μm），以甲醇-水（4：1，v/v）为流动相，成功实现了非对映异构体的分离和测定。紫外检测波长为270 nm。（+）-里卡林A标准曲线的线性范围为0.25 - 150.00μg/mL，异里卡林A标准曲线的线性范围为0.10 - 75.00μg/mL。（+）-里卡林A的检测限和定量下限分别为0.05和0.25μg/mL，异里卡林A的检测限和定量下限分别为0.05和0.10μg/mL。该测定方法成功应用于大鼠血浆中（+）-里卡林A和异里卡林A非对映异构体的药代动力学研究。
