液相色谱-串联质谱法：一种测定大鼠脑核中脱氢二异丁香酚的灵敏方法。
Liquid Chromatography with Tandem Mass Spectrometry: A Sensitive Method for the Determination of Dehydrodiisoeugenol in Rat Cerebral Nuclei.
作者信息
Zhang You-Bo, Yang Xin-Bao, Yang Xiu-Wei, Xu Wei, Li Fei, Gonzezal Frank J
机构信息
State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, No. 38, Xueyuan Road, Haidian District, Beijing 100191, China.
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Molecules. 2016 Mar 9;21(3):321. doi: 10.3390/molecules21030321.
A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is developed for the quantification of dehydrodiisoeugenol (DDIE) in rat cerebral nuclei after single intravenous administration. DDIE and daidzein (internal standard) were separated on a Diamonsil™ ODS C18 column with methanol-water containing 0.1% formic acid (81:19, v/v) as a mobile phase. Detection of DDIE was performed on a positive electrospray ionization source using a triple quadrupole mass spectrometer. DDIE and daidzein were monitored at m/z 327.2→188.0 and m/z 255.0→199.2, respectively, in multiple reaction monitoring mode. This method enabled quantification of DDIE in various brain areas, including, cortex, hippocampus, striatum, hypothalamus, cerebellum and brainstem, with high specificity, precision, accuracy, and recovery. The data herein demonstrate that our new LC-MS/MS method is highly sensitive and suitable for monitoring cerebral nuclei distribution of DDIE.
建立了一种新的液相色谱-串联质谱（LC-MS/MS）方法，用于定量单次静脉给药后大鼠脑核中的去氢二异丁香酚（DDIE）。DDIE和大豆苷元（内标）在Diamonsil™ ODS C18柱上分离，以含0.1%甲酸的甲醇-水（81:19，v/v）为流动相。使用三重四极杆质谱仪在正电喷雾电离源上对DDIE进行检测。在多反应监测模式下，分别在m/z 327.2→188.0和m/z 255.0→199.2处监测DDIE和大豆苷元。该方法能够对包括皮质、海马体、纹状体、下丘脑、小脑和脑干在内的各个脑区中的DDIE进行定量，具有高特异性、精密度、准确度和回收率。本文数据表明，我们新的LC-MS/MS方法高度灵敏，适用于监测DDIE的脑核分布。
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