Phillips T M, Dickens B F
Immunochemistry Laboratory, The George Washington University Medical Center, Washington, DC 20037, USA.
Electrophoresis. 1998 Nov;19(16-17):2991-6. doi: 10.1002/elps.1150191632.
An immunoaffinity capillary electrophoresis (ICE) system for rapidly quantifying recombinant cytokines in human body fluids has been developed. Cytokines within biological fluids were labeled with a red light emitting fluorochrome and injected into the capillary. Selected cytokines were captured by immobilized antibodies on the internal surface of the capillary, and held while unbound materials were purged. The cytokines were then eluted electrophoretically in acidic buffer. Individual cytokine peaks were detected by on-line laser-induced fluorescence detection coupled to a computerized fiber-optic spectrometer, and analyzed by integration of the eluted peaks. The comparison of the results of ICE to routine assays used for these cytokines demonstrates that ICE provides a fast and accurate procedure for defining these cytokines in complex biological samples. Immunoaffinity separations can be used for any material to which a specific antibody can be raised, making this procedure applicable to a wide range of molecules of biomedical interest.
已开发出一种用于快速定量人体体液中重组细胞因子的免疫亲和毛细管电泳（ICE）系统。生物体液中的细胞因子用发红光的荧光染料标记，然后注入毛细管。选定的细胞因子被固定在毛细管内表面的抗体捕获，在冲洗未结合物质时保持固定状态。然后在酸性缓冲液中通过电泳洗脱细胞因子。通过与计算机化光纤光谱仪联用的在线激光诱导荧光检测来检测各个细胞因子峰，并通过对洗脱峰进行积分来分析。将ICE的结果与用于这些细胞因子的常规检测方法进行比较表明，ICE为在复杂生物样品中鉴定这些细胞因子提供了一种快速且准确的方法。免疫亲和分离可用于任何能够产生特异性抗体的物质，使得该方法适用于广泛的具有生物医学意义的分子。
