Wang Joseph, Chatrathi Madhu Prakash, Collins Greg E
Departments of Chemical Engineering and Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287-5001, USA.
Anal Chim Acta. 2007 Feb 28;585(1):11-6. doi: 10.1016/j.aca.2006.12.001. Epub 2006 Dec 9.
A miniaturized capillary electrophoretic (CE) microchip device for the simultaneous measurements of lactate and glucose is described. The new microchip bioassay protocol integrates an electrophoretic separation of lactate and glucose, post-column enzymatic reactions of these metabolites with their respective oxidase enzymes, and an amperometric (anodic) detection of enzymatically-liberated hydrogen peroxide at a gold-coated thick-film carbon detector. Factors influencing the response have been examined and optimized, and the analytical performance has been characterized. Applicability of the microchip assay to clinical samples, such as serum and blood, is demonstrated. The microchip protocol obviates cross enzymatic reactions and interferences from major oxidizable constituents common to dual glucose-lactate enzyme electrodes. Such ability to rapidly separate and quantitate lactate and glucose on a small microchip platform should find important clinical and biotechnological applications.
本文描述了一种用于同时测量乳酸和葡萄糖的小型化毛细管电泳（CE）微芯片装置。新的微芯片生物测定方案集成了乳酸和葡萄糖的电泳分离、这些代谢物与各自氧化酶的柱后酶促反应，以及在金涂层厚膜碳检测器上对酶促释放的过氧化氢进行安培（阳极）检测。研究并优化了影响响应的因素，并对分析性能进行了表征。证明了微芯片测定法对血清和血液等临床样本的适用性。该微芯片方案避免了交叉酶促反应以及双葡萄糖 - 乳酸酶电极常见的主要可氧化成分的干扰。这种在小型微芯片平台上快速分离和定量乳酸和葡萄糖的能力应具有重要的临床和生物技术应用。
