Lodi T, Donnini C, Ferrero I
Institute of Genetics, University of Parma, Italy.
J Gen Microbiol. 1991 May;137(5):1039-44. doi: 10.1099/00221287-137-5-1039.
Catabolite repression by galactose was investigated in several strains of Saccharomyces cerevisiae grown on different carbon sources. Galactose repressed as much as glucose; raffinose was less effective. Full derepression was achieved with lactate. The functions tested were L-lactate ferricytochrome c oxidoreductase, NAD-glutamate dehydrogenase, and respiration. Galactose repression was observed only in the GAL4 but not in the gal4 strain. The presence of multiple copies of the GAL4 gene enhanced the repression by galactose. Different alleles of the GAL4 gene and the copy number did not affect glucose repression.
在以不同碳源生长的几株酿酒酵母中研究了半乳糖的分解代谢物阻遏作用。半乳糖的阻遏作用与葡萄糖相当；棉子糖的作用较弱。乳酸可实现完全去阻遏。所测试的功能包括L-乳酸铁细胞色素c氧化还原酶、NAD-谷氨酸脱氢酶和呼吸作用。仅在GAL4菌株中观察到半乳糖阻遏作用，而在gal4菌株中未观察到。GAL4基因的多个拷贝增强了半乳糖的阻遏作用。GAL4基因的不同等位基因和拷贝数不影响葡萄糖阻遏作用。
