Donnini C, Goffrini P, Rossi C, Ferrero I
Institute of Genetics, University of Parma, Italy.
Microbiologica. 1990 Oct;13(4):283-95.
Two carbon catabolite repression mutants of S. cerevisiae were isolated and characterized. In spite of the selection procedure (red colonies after tetrazolium overlay at high glucose concentration) the mutants exhibited a respiration which was as repressed as that of the parental strain or even more repressed. When grown at high glucose concentration the mutants display hyper-repression of cytochrome aa3 and of certain mitochondrial enzymes (L- and D-lactate dehydrogenases) but not of others (malate dehydrogenase, succinate dehydrogenase), indicating the existence of separate control sites for the different genes involved in the mitochondrial biogenesis. The data obtained pointed out that the same mutation affects both repression and derepression. In addition, the mutation(s) give rise to the complete derepression of the cytoplasmic enzyme NAD-glutamate dehydrogenase at 10% glucose whereas the enzyme is normally repressed at 3% glucose. The results of the genetic analysis indicate the mitochondrial nature of the mutation(s).
分离并鉴定了酿酒酵母的两个碳分解代谢物阻遏突变体。尽管采用了选择程序（在高葡萄糖浓度下经四氮唑覆盖后出现红色菌落），但这些突变体的呼吸作用受到的抑制程度与亲本菌株相同，甚至受到更强的抑制。当在高葡萄糖浓度下生长时，这些突变体表现出细胞色素aa3和某些线粒体酶（L-和D-乳酸脱氢酶）的超阻遏，但其他酶（苹果酸脱氢酶、琥珀酸脱氢酶）则没有，这表明参与线粒体生物发生的不同基因存在独立的调控位点。所获得的数据指出，相同的突变会影响阻遏和去阻遏。此外，该突变会导致细胞质酶NAD-谷氨酸脱氢酶在10%葡萄糖浓度下完全去阻遏，而该酶在3%葡萄糖浓度下通常受到阻遏。遗传分析结果表明该突变具有线粒体性质。
