Kusek Justyna, Seremak-Mrozikiewicz Agnieszka, Drews Krzysztof, Mikołajczak Przemysław, Czerny Bogusław, Maciejewska Monika, Bogacz Anna, Derebecka-Hołysz Natalia, Barlik Magdalena, Mrozikiewicz Przemysław M
Instytut Roślin i Przetworów Zielarskich w Poznaniu.
Ginekol Pol. 2008 Jun;79(6):426-31.
Proinflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha), involved into osteoclastogenesis and responsible for bone resorption process, participate in the pathogenesis of the osteoporosis. In vitro studies have shown that single nucleotide polymorphisms of IL-6 and TNF-alpha genes could influence the transcription process of the genes and the cytokines level.
Assessment of the influence of IL-6 and TNF-alpha gene polymorphisms on bone mineral density (BMD) and evaluation of their connection with osteoporosis prevalence in women from Wielkopolska region.
In the group of 267 postmenopausal women (average age 58.5+/-5.9 years, average age of last period 49.8+/-3.9 years) bone mineral density in lumbar spine (L2-L4) was performed using dual energy X-ray absorptiometry (DXA). Genotypes frequencies were determined by polymerase chain reaction with restriction fragment length polymorphism (PCR/RFLP) using restriction enzymes Lwe I and Faq I, respectively. The connection between the polymorphisms of investigated genes and body mass index, age of menarche and menopause and length of reproductive age had been analyzed as well.
No statistically significant association was found between examined genetic factors and the value of bone mineral density in the investigated group of postmenopausal women. The frequencies of investigated genotypes were in compliance with Hardy-Weinberg equilibrium. The correspondence between evaluated clinical parameters and IL-6 i TNF-alpha genotypes frequencies has not been proven.
The -174G/C polymorphism in the IL-6 and -308G/A polymorphism in TNF-alpha genes have no influence on bone mineral density value (BMD) in the investigated population of women from Wielkopolska region.
促炎细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子α（TNF-α）参与破骨细胞生成并负责骨吸收过程，它们参与骨质疏松症的发病机制。体外研究表明，IL-6和TNF-α基因的单核苷酸多态性可能影响基因的转录过程和细胞因子水平。
评估IL-6和TNF-α基因多态性对骨密度（BMD）的影响，并评估它们与大波兰地区女性骨质疏松症患病率的关系。
对267名绝经后女性（平均年龄58.5±5.9岁，最后一次月经的平均年龄49.8±3.9岁）进行腰椎（L2-L4）骨密度检测，采用双能X线吸收法（DXA）。分别使用限制性内切酶Lwe I和Faq I，通过聚合酶链反应-限制性片段长度多态性（PCR/RFLP）确定基因型频率。还分析了所研究基因的多态性与体重指数、初潮和绝经年龄以及生育年龄长度之间的关系。
在被研究的绝经后女性组中，未发现所检测的遗传因素与骨密度值之间存在统计学上的显著关联。所研究基因型的频率符合哈迪-温伯格平衡。尚未证实所评估的临床参数与IL-6和TNF-α基因型频率之间的对应关系。
IL-6基因中的-174G/C多态性和TNF-α基因中的-308G/A多态性对大波兰地区被研究女性人群的骨密度值（BMD）没有影响。
