Seino Nobutaka, Matsumoto Kotaro, Hayakawa Daisuke, Suzuki Masatsugu, Hata Hirosi, Kondo Seiji, Yokoyama Naoaki, Inokuma Hisashi
Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Jpn J Infect Dis. 2008 Jul;61(4):315-7.
Rickettsial infection of cattle and sika deer from a pastureland in Hidaka District, Hokkaido, Japan was examined by serological and molecular methods. Serum samples from 8 of 83 (9.6%) cattle reacted with Rickettsia helvetica antigens in an IFA test, with titers ranging from 1:160 to 1:640, while serum samples from 15 of 22 (68.2%) deer were positive for R. helvetica, with titers ranging from 1:80 to 1:640. In a genus-specific nested PCR based on gltA, no cattle were positive for Rickettsia, while 14 of 22 (63.3%) samples obtained from deer tested positive. Sequence analysis revealed that positive samples from sika deer showed 100% nucleotide sequence identity with the known sequence of Rickettsia asiatica.
采用血清学和分子方法对日本北海道日高地区一片牧场上的牛和梅花鹿的立克次氏体感染情况进行了检测。在间接荧光抗体试验中，83头牛中有8头（9.6%）的血清样本与瑞士立克次氏体抗原发生反应，滴度范围为1:160至1:640，而22头鹿中有15头（68.2%）的血清样本对瑞士立克次氏体呈阳性反应，滴度范围为1:80至1:640。在基于柠檬酸合酶基因（gltA）的属特异性巢式聚合酶链反应（PCR）中，没有牛的样本检测出立克次氏体阳性，而从鹿身上采集的22份样本中有14份（63.3%）检测呈阳性。序列分析表明，梅花鹿的阳性样本与亚洲立克次氏体的已知序列具有100%的核苷酸序列同一性。
