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日本北海道一处有梅花鹿出没的牧场中牛的牛无浆体和嗜吞噬细胞无形体感染的分子调查

Molecular survey for Anaplasma bovis and Anaplasma phagocytophilum infection in cattle in a pastureland where sika deer appear in Hokkaido, Japan.

作者信息

Seino Nobutaka, Hayakawa Daisuke, Suzuki Masatsugu, Hata Hiroshi, Kondo Seiji, Matsumoto Kotaro, Yokoyama Naoaki, Inokuma Hisashi

机构信息

Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.

出版信息

Jpn J Infect Dis. 2009 Jan;62(1):73-5.

PMID:19168967
Abstract

Anaplasma bovis and Anaplasma phagocytophilum infection were examined by species-specific nested polymerase chain reaction in cattle in a pastureland where sika deer appear in Hokkaido, Japan. Of the 78 cattle examined, 12 (15%) and 1 (1%) tested positive for infection by A. bovis and A. phagocytophilum, respectively. One cattle tested positive for both. A. phagocytophilum infection rates were significantly lower in cattle than in sika deer (46%), but the infection rate by A. bovis was not significantly different between cattle (15%) and sika deer (23%). The strain of A. phagocytophilum detected in this study may possess significantly lower virulence or infectivity in cattle hosts. No clinical symptoms were recorded in the positive cattle, and morulae were not detected in the blood smears.

摘要

在日本北海道一个有梅花鹿出没的牧场上,通过种特异性巢式聚合酶链反应对牛的牛无形体和嗜吞噬细胞无形体感染情况进行了检测。在所检测的78头牛中,分别有12头(15%)和1头(1%)牛无形体和嗜吞噬细胞无形体感染检测呈阳性。有1头牛两种感染检测均呈阳性。嗜吞噬细胞无形体在牛中的感染率显著低于梅花鹿(46%),但牛无形体在牛(15%)和梅花鹿(23%)中的感染率没有显著差异。本研究中检测到的嗜吞噬细胞无形体菌株在牛宿主中的毒力或感染力可能显著较低。阳性牛未记录到临床症状,血涂片也未检测到桑葚体。

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