Okoń Krzysztof
Department of Pathomorphology, Collegium Medicum, Jagiellonian University, Kraków.
Pol J Pathol. 2008;59(1):15-20.
Glypicans are core proteins of heparan sulfate proteoglycans that have numerous functions. Glypican-3 (GPC3) expression has been detected to be altered in several tumors, including hepatocellular carcinoma, breast carcinoma, and embryonic malignancies. To date, no information on GPC3 status in renal cell carcinoma is available. The material for the study consisted of 625 cases of renal tumors diagnosed at our institution. Tissue microarrays were constructed using all acceptable quality tissue blocks. Immunohistochemistry for GPC3 was performed with 1G12 antibody (BioMosaics). We found strong positive staining in 15 cases, moderate in 4 and weak in 68. The reactivity was particularly evident in chromophobe renal cell carcinomas (32/40). Our findings are of note in cases when GPC3 may be used in differential diagnosis of tumors of uncertain primary location.
磷脂酰肌醇蛋白聚糖是硫酸乙酰肝素蛋白聚糖的核心蛋白，具有多种功能。已检测到磷脂酰肌醇蛋白聚糖-3（GPC3）在包括肝细胞癌、乳腺癌和胚胎恶性肿瘤在内的多种肿瘤中表达发生改变。迄今为止，尚无关于肾细胞癌中GPC3状态的信息。本研究材料包括在我们机构诊断的625例肾肿瘤病例。使用所有质量合格的组织块构建组织微阵列。用1G12抗体（BioMosaics）进行GPC3免疫组织化学检测。我们发现15例强阳性染色，4例中度阳性，68例弱阳性。这种反应性在嫌色性肾细胞癌中尤为明显（32/40）。当GPC3可用于鉴别原发部位不明的肿瘤时，我们的发现值得关注。
