Andisheh-Tadbir Azadeh, Goharian Amir Saeed, Ranjbar Mohammad Ali
Oral and Dental Disease Research Center, Dept. of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Undergraduate Student, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
J Dent (Shiraz). 2020 Jun;21(2):141-146. doi: 10.30476/DENTJODS.2019.84541.1089.
Oral squamous cell carcinoma (OSCC) is a malignant neoplasm that affects the structures or tissues of mouth. Early diagnosis of these tumors is important to improve the outcome of treatment. Therefore, using pathological techniques based on molecular markers may be useful for optimal diagnosis and treatment. Glypican-3 (GPC3) is involved in regulation of cell proliferation and morphogenesis and is abundant during embryogenesis and organogenesis but is limited in most of adult tissues. GPC3 overexpression has a role in carcinogenesis.
The aim of the present study was to investigate GPC3 expression in the non-neoplastic oral epithelium and oral squamous cell carcinoma.
In this cross-sectional study, 45 patients with OSCC (30 males and 15 females) with a mean age of 52.3 selected from Oral Pathology Department of Shiraz Dental School were enrolled. The control group was consisted of 15 cases of normal oral epithelium. Glypican-3 expression was assessed by using immunohistochemical methods.
Non neoplastic tissues were GPC3 negative. Frequency of GPC3 positivity in tumoral tissues was recorded as 73.3% (33 cases) which was significantly higher than non-neoplastic tissues (< 0.001).The clinicopathologic features of GPC3 expression demonstrated no association with clinicopathologic parameters except tumor size.
GPC3 was over expressed at protein level in oral squamous cell carcinoma, but its potential use for diagnostic and therapeutic purposes requires further investigation.
口腔鳞状细胞癌（OSCC）是一种影响口腔结构或组织的恶性肿瘤。早期诊断这些肿瘤对于改善治疗结果很重要。因此，使用基于分子标志物的病理技术可能有助于实现最佳诊断和治疗。磷脂酰肌醇蛋白聚糖-3（GPC3）参与细胞增殖和形态发生的调节，在胚胎发生和器官发生过程中含量丰富，但在大多数成人组织中含量有限。GPC3的过表达在致癌过程中起作用。
本研究的目的是调查GPC3在非肿瘤性口腔上皮和口腔鳞状细胞癌中的表达情况。
在这项横断面研究中，纳入了从设拉子牙科学院口腔病理科选取的45例OSCC患者（30例男性和15例女性），平均年龄为52.3岁。对照组由15例正常口腔上皮病例组成。采用免疫组织化学方法评估磷脂酰肌醇蛋白聚糖-3的表达。
非肿瘤组织GPC3呈阴性。肿瘤组织中GPC3阳性率为73.3%（33例），显著高于非肿瘤组织（<0.001）。GPC3表达的临床病理特征显示，除肿瘤大小外，与临床病理参数无关联。
GPC3在口腔鳞状细胞癌中蛋白水平过表达，但其在诊断和治疗方面的潜在用途需要进一步研究。
