Kalathingal Sajitha Menon, Moore Stephanie, Kwon Soon, Schuster George S, Shrout Michael K, Plummer Kevin
Oral Health and Diagnostic Sciences, School of Dentistry, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912-1241, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Feb;107(2):279-82. doi: 10.1016/j.tripleo.2008.05.025. Epub 2008 Jul 25.
The objective of this study was to determine if phosphor plates used in predoctoral clinics are microbiologically contaminated and to identify the source of contamination.
Forty-five of 300 phosphor plates (15%) were randomly selected for examination. The plates were pressed into individual blood agar plates, were incubated using standard techniques at 37 degrees C, and were monitored for 72 hours. The number, size, distribution, and variety of resulting colonies were noted. A representative of each type of colony was selected to be Gram stained using the standard technique.
Of the plates, 42.2% were uncontaminated, 57.8% yielded bacterial colonies, and 15.6% of those colonies demonstrated hemolytic growth. The hemolytic growth included combined alpha and beta hemolysis and beta only hemolysis. Six colonies were gram-positive rods and 7 were gram-positive cocci.
Meticulous infection-control techniques are inevitable and continuous reinforcement and training for staff and students are mandatory. Periodic gas sterilization of phosphor plates may be necessary.
本研究的目的是确定博士前临床使用的磷光板是否受到微生物污染，并确定污染源。
从300块磷光板中随机选取45块（15%）进行检查。将这些磷光板压入单独的血琼脂平板中，采用标准技术在37℃下培养，并监测72小时。记录所得菌落的数量、大小、分布和种类。每种菌落类型选取一个代表，采用标准技术进行革兰氏染色。
在这些磷光板中，42.2%未受污染，57.8%产生细菌菌落，其中15.6%的菌落表现为溶血生长。溶血生长包括α和β溶血合并以及仅β溶血。6个菌落为革兰氏阳性杆菌，7个为革兰氏阳性球菌。
细致的感染控制技术是必不可少的，对工作人员和学生进行持续强化和培训是必要的。磷光板可能需要定期进行气体灭菌。
