Hunter Allison, Kalathingal Sajitha, Shrout Michael, Plummer Kevin, Looney Stephen
Radiology Oral Health and Diagnostic Sciences, Georgia Regents University, College of Dental Medicine, Augusta, GA, USA.
Department of Oral Rehabilitation, Georgia Regents University, College of Dental Medicine, Augusta, GA, USA.
Imaging Sci Dent. 2014 Jun;44(2):149-54. doi: 10.5624/isd.2014.44.2.149. Epub 2014 Jun 11.
This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates.
Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine®, Decapinol®, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37℃ for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed.
The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates.
The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.
本研究评估了三种抗菌漱口水在减少光激励荧光板（PSP）上微生物生长方面的有效性。
在进行全口放射检查（FMX）之前，要求受试者用三种测试漱口水之一（李施德林漱口水、德卡匹诺漱口水或0.12%氯己定口腔漱口水）漱口，或不进行漱口。在完成FMX扫描后，从每个受试者处收集四个PSP板并放入无菌容器中。使用火焰消毒的镊子将PSP板转移到血琼脂平板（5%绵羊血琼脂）上。血琼脂平板在37℃下培养长达72小时。每批培养时都设置一个环境对照血琼脂平板。此外，作为对照，将25个气体灭菌的PSP板接种到血琼脂上并进行分析。
氯己定组每平板的细菌菌落平均数最低，其次是德卡匹诺组、李施德林组和不漱口阴性对照组。只有氯己定组和李施德林组有显著差异（p = 0.005）。25个气体灭菌对照平板和环境对照血琼脂平板均未观察到生长。
氯己定组的细菌菌落平均数最低，其次是德卡匹诺组、李施德林组和不漱口组。然而，仅在李施德林组发现了统计学上的显著差异。需要进一步研究以测试更高浓度（0.2%）或更长暴露时间（连续两次30秒漱口）是否有助于进一步减少氯己定对PSP板的污染。
